Abstract:

AIM： To in vitro observe the cell compatibility and release of biodegradable poly （lactide-co-glycolide acid） （PLGA） scaffold integrated with nerve growth factor （NGF）. METHODS： The experiment was carried out in the Laboratory of Injury Repairs, Beijing Institute of Neuresurgery Affiliated to Capital Medical University from May to September in 2005. ①The production of PLGA was made by technology of patent （200510011350.9）. Schwann cells and neural stem cells were cultured within PLGA scaffold in vitro. ②The growth of Schwann cells and neural stem cells were observed by scanning electron microscope; NGF was integrated into PLGA by tissue engineering technology, 1 μg NGF for 0.02 g PLGA （dry weight）; The release concentration of NGF was tested with ELISA. RESULTS：①Scanning electron microscope examination： Plenty of meshes on T-sect and straight channels on longitudinal section were observed on PLGA. ②Schwann cells and neural stem cells grew well and touched tightly in PLGA scaffold.③The concentrations of NGF reached the peak amplitude on the 4^th day, and released steadily on the 7^th day with the value was 100 μg/L. CONCLUSION： ①PLGA scaffold has well biological compatibility and cellular affinity with Schwann cells and neural stem cells.②With the ratio of 1μg NGF in 0.02 g PLGA, NGF can constantly release on the level of 100 μg/L, which displays the biological activity.[著者文摘]