学术
摘 要:
目的：分析硫色曲霉产木聚糖酶的酶学性质。方法：利用RT-PCR克隆了硫色曲霉的木聚糖酶基因州，构建了该基因的原核表达载体，并获得了诱导表达。结果：纯化的木聚糖酶的最适催化温度为50℃，最适反应pH值为2．4，在30-50℃保温30min对酶活性没有大的影响。结论：木聚糖酶在低pH值下的催化活性使之在饲料工业中具有较好的应用前景。[著者文摘]
文章出处:
《生物技术通讯》-2006年17卷6期 -878-881页
栏目信息:
文献标识码:
A
文章编号:
1009-0002（2006）06-0878-04
Cloning, Expression and Enzyme Characterization Analysis of Aspergillus sulphureus Xylanase Gene xynA
CAO Yun-he, CHEN Xiao-ling, HE Ping-li, LU Wen-qing （State Key Laboratory of Animal Nurition, China Agricultural University, Beijing 100094, China）
Abstract:
Objective： To analysis enzyme characterization of the xylanase produced by Aspergillus sulphureus. Methods： The xylanase gene xynA frome A.sulphureus was amplified by RT-PCR, and expressed induced with IPTG in Escherichla coll. Results： The purified enzyme was optimally active at 50℃ and pH2.4. No obvious influence on the enzyme activity was observed when it has been incubated for 30 min under 30-50℃ bath. Conclusion： The acidic activity of the purified xylanase makes this enzyme attractive for use in feed industrial application.[著者文摘]
Key words:
Aspergillus sulphureus; xylanase; prokaryotic expression; characterization analysis
基金资助:
国家科技部动物营养与饲料加工创新群体科学基金项目（30121004）
更多>>免费文章
cqvip.com