学术
摘 要:
目的比较PCR和反相被动乳胶凝集（RPLA）法检测金黄色葡萄球菌肠毒素A～D型的效果。方法对9株金黄色葡萄球菌分别用PCR和RPLA法检测金黄色葡萄球菌肠毒素A～D型，并以标准株作对照质控。结果两种方法的检测结果一致，9株金黄色葡萄球菌中有4株检出肠毒素A型，均未检出肠毒素B、C和D型。结论PCR法较适合基层实验室应用于检测金黄色葡萄球菌肠毒素。[著者文摘]
文章出处:
《中国热带医学》-2007年7卷11期 -1975-1976页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1009-9727（2007）11-1975-02
Comparison of results of identification of Staphylococcus aureus enterotoxins A - D by PCR and RPLA.
Lü Chao - yang. （Huangzhou District Center for Disease Control and Prevention of Huanggang City, Huanggang 438000, Hubei, P. R. China）
Abstract:
Objective To compare the results of idendentificafion of Staphylococcus aureus enterotoxins A - D by PCR and reversed passive latex agglutination （ RPLA）. Methods Staphylococcus aureus enterotoxins A - D of 9 isolates of Staphylococcus aureus were identied by PCR and RPLA respectively. Results The results of the two methods were almost identical. Four strains of Staphylococcus aureus enterotoxins A were identified from 9 isolates. Staphylococcus aureus enterotoxins B, C and D were not detected. Conclusion PCR is suitable for identification of Staphylococcus aureus enterotoxins in general laboratory.[著者文摘]
Key words:
PCR; RPLA; Staphylococcus aureus ; Enterotoxins
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