学术
摘 要:
目的了解本地区儿童患者临床分离的产超广谱β-内酰胺酶（ESBLs）大肠埃希菌的耐药基因型别和耐药性的变化情况。方法收集自儿童患者分离鉴定的大肠埃希菌株1327株，通过K-B法作药敏试验，采用美国NCCLS1999年推荐的抑制剂增强纸片法确认产ESBLs株，并对ESBLs进行初步基因分型。结果检出产ESBLs菌株702株，检出率为52．9％，产ESBLs大肠埃希菌对青霉素类、氨曲南及头孢菌素类抗生素耐药率为55．5％。95．0％；PCR初步分型结果，单独携带TEM型基因的占56．7％，单独携带SHV型基因的占15．0％，携带两种基因的占25．8％。结论产ESBLs大肠埃希菌检出率逐年上升，具有多重耐药的特点；产ESBLs大肠埃希菌主要携带TEM型和SHV型β-内酰胺酶基因，其中绝大部分产TEM型β-内酰胺酶。[著者文摘]
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文章出处:
《中国热带医学》-2007年7卷11期 -2009-2010页
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分 类 号:
文献标识码:
A
文章编号:
1009-9727（2007）11-2009-02
Analysis of drug resistance and gen typing of ESBLs - producing Escherichia coli isolated from infants.
SHI Sheng, XIONG Yan. （ Department of Clinical Laboratory, Jiangmen Municipal People＇ s Hospital, Jiangmen 529000, Guangdong, P.R. China）
Abstract:
Objective To investigate current resistant status and genetype of ESBLs - producing Escherichia coli in child patients. Methods There were 1 327 Escherichia coli strains isolated in clinic, and the ESBLs - predueing isolates were identified by K- B method. The DNA of ESBLs - predueing Escherichia coli were genetyped by polymerase chain reaction （PCR）. Results There were 702 ESBLs- predueing isolates with a positive rate of 52.9%. The resistant rates of ESBLs - producing Escherichia coli isolates to penieillins,aztreonam and the third generation eephalosporins were from 55.5% to 100%. The results of PCR showed that the rates of TEM type, SHV type and TEM with SHV type in ESBLs - producing Escherichia coli isolates were 56.7%, 15.0% and 25.8 %, respectively. Conclusion The incidence of ESBLs - predueing Escherichia coli isolates was increased year by year, and they were multi - resistant. Most of the ESBLs - predueing Escherichia coli isolates carry TEM related genes or/and SHV related genes, and the TEM related genes were the most.[著者文摘]
Key words:
Extended speetrum β- laetamases（ESBLs） ; Escherichia coli; Resistance; Gene typing
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