学术
海水淹溺兔肺泡巨噬细胞TNF-α mRNA的动态表达及地塞米松干预作用
顾兴 金发光 傅恩清 刘同刚 刘静莉 孙亚妮
第四军医大学唐都医院呼吸内科,西安710038
摘 要:
目的：观察海水淹溺后家兔肺泡巨噬细胞内TNF-α mRNA的动态表达规律，并探讨地塞米松干预对TNF-α mRNA表达的影响。方法：采用气管切开插入塑料导管、向气管内灌海水4ml／kg，双肺自主通气的方法模拟海水淹溺造成急性肺损伤模型。于淹溺后0min、30min、60min、90min、120min五个观察时间点进行血气分析，并采集肺组织，逆转录一聚合酶链反应（RT-PCR）检测肺泡巨噬细胞中TNF-α mRNA的表达。结果：海水淹溺兔急性肺损伤各时相点肺组织中性粒细胞大量浸润，淹溺后TNF-α mRNA表达显著增高，90分钟后达最高峰，而地塞米松可使之降低（P〈0．01）。结论：海水淹溺急性肺损伤可能与肺泡巨噬细胞内TNF-α mRNA高表达有关。地塞米松可降低TNF-α mRNA的高表达，减轻肺损伤程度。[著者文摘]
文章出处:
《现代生物医学进展》-2007年7卷11期 -1631-1634页
栏目信息:
文献标识码:
A
文章编号:
1673-6273（2007）11-1631-04
Effects of Dexamethasone Injection on the Expression of TNF-α mRNA in Pulmonary Alveolar Macrophages（AM） of Rabbits with Sea Water Drowning
GU Xing, JIN Fa-guang, FU En-qing, LIU Tong-gang, LIU Jing-li, SUN Ya-ni （Department of respiratory disease, The second hospital of Forth Military Medical University, XI ＇AN 710038, China ）
Abstract:
Objective： To observe the dynamic change in the expression of TNF-α mRNA expression in pulmonary alveolar macrophage （AM） of in rabbits drowned by with sea water drowning, and explore the effects of dexamethasone injection on the expression of TNF-α mRNA. Methods： The drowning model was established by pouring sea water （4ml/kg） to the trachea of rabbits using plastic tube during spontaneous breathing of bilateral lung auto ventilated, to simulate the process of drowning. The expression of TNF-α mRNA in pulmonary alveolar macrophages of rabbits was examined by reverse-transcription polymerase chain reaction （RT-PCR） after being drowned 0min, 30min, 60 rain, 90 min and 120min respectively. Lung tissues were examined by histopathology, and the blood gas analysis was determinated. Results： ARer drowning, the infiltration of PMN and the expression of TNF-α mRNA increased significantly, and reached highest after 90 minutes drowning. Dexamethasone inhibitsthe expression of TNF-α mRNA and extenuates acute lung injury. Conclusion： For sea water respiratory distress syndrome （SW-RDS）, acute lung damage at early stage might be related to the high expression of TNF-α mRNA in AM. Dexamethasone can significantly decrease the expression of TNF-α mRNA in the alveolar macrophage （P〈0.01 ）, and extenuate acute lung injury.[著者文摘]
Key words:
Sea water drowning; Acute lung iniury; Alveolar macroohage （AM）; Dexamethasone, TNF-α
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