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DNA purification and genetyping: Based on multifunc-tional nanobeads

《中国科学通报:英文版》2004年 第9期 | XIExin ZHANGXu GAOHuafang ZHANGHuan CHENDepu CHENGJing FEIWeiyang   StateKeyLaboratoryofExtractionSeparationEngineering Depart-mentofChemicalEngineering TsinghuaUniversity Beijing100084 China FacultyofScience SichuanAgriculturalUniversity Ya'an625014 China DepartmentofBiologicalSciencesandBiotechnology TsinghuaUniversity Beijing100084 China DepartmentofChemicalEngineering MassachusettsInstituteofTechnology CambridgeMA02139 USA DepartmentofChemistry TsinghuaUniversity Beijing100084 China NationalEngineeringResearchCenterforBeijingBiochipTechnol-ogy Beijing102206 China
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摘 要:In this report, a universal protocol for extract-ing genomie DNA from whole blood, saliva, and bacterialculture by using magnetic nanobeads as solid-phase absor-bents was presented. The enrichment of target cells and ad-sorption of DNA have been functionally integrated onto thesurfaces of the earboxyl-modified magnetic nano-beads, andthe DNA segments bound on the surface of the beads can bedirectly used as PCR templates to amplify a target geue. ThePCR products were applied to an oligonueleotide array toperform gene typing. The protocol proves to be simple, rapid,biologically and chemically nonhazardous, and promising forthe mierofabrieation of DNA preparation chip.
【分 类】【医药、卫生】 > 临床医学 > 诊断学【生物科学】 > 分子生物学 > 基因工程(遗传工程)
【关键词】 多功能纳米床 DNA纯化 染色体组DNA 微片 PCR 固相吸收 显微完全分析系统 诊断 R44
【出 处】 《中国科学通报:英文版》2004年 第9期 886-889页 共4页
【收 录】 中文科技期刊数据库