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Single-cell transcriptome analysis reveals widespread monoallelic gene expression in individual rice mesophyll cells

《科学通报:英文版》2017年 第19期 | Yingying Han Xiao Chu Haopeng Yu Ying-Ke Ma Xiu-Jie Wang Wenfeng Qian Yuling Jiao   State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Key Laboratory of Genetic Network Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 Chin University of Chinese Academy of Sciences Beijing 100049 China
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摘 要:Monoallelic gene expression refers to the phenomenon that all transcripts of a gene in a cell are expressed from only one of the two alleles in a diploid organism. Although monoallelic gene expression has been occasionally reported with bulk transcriptome analysis in plants, how prevalent it is in individual plant cells remains unknown. Here, we developed a single-cell RNA-seq protocol in rice and investigated allelic expression patterns in mesophyll cells of indica(93-11) and japonica(Nipponbare) inbred lines, as well as their F1 reciprocal hybrids. We observed pervasive monoallelic gene expression in individual mesophyll cells, which could be largely explained by stochastic and independent transcription of two alleles. By contrast, two mechanisms that were proposed previously based on bulk transcriptome analyses, parent-oforigin effects and allelic repression, were not well supported by our data. Furthermore, monoallelically expressed genes exhibited a number of characteristics, such as lower expression levels, narrower H3K4me3/H3K9ac/H3K27me3 peaks, and larger expression divergences between 93-11 and Nipponbare. Taken together, the development of a single-cell RNA-seq protocol in this study offers us an excellent opportunity to investigate the origins and prevalence of monoallelic gene expression in plant cells.
【分 类】【生物科学】 > 分子生物学 > 基因工程(遗传工程) > 基因的表达
【关键词】 基因表达模式 等位基因 基因转录 叶肉细胞 单细胞 水稻 组分 个体
【出 处】 《科学通报:英文版》2017年 第19期 1304-1314页 共11页
【收 录】 中文科技期刊数据库