人SOD基因的克隆与原核表达
宋凯[1] 宋继梅[2]
[1]徐州生物工程高等职业学校,江苏徐州221006 [2]西北农林科技大学生物工程研究所,陕西杨陵712100
摘 要:
超氧化物歧化酶(SOD)是机体内超氧自由基的天然清除剂,在抗衰老、抗辐射、抗逆境,消炎、抑制肿瘤和癌症,以及自身免疫疾病的治疗方面有很重要的作用。我国目前主要从血液中提取SOD,有关重组人SOI)的研究较少。文章通过RTPCR方法克隆得到人SOD基因,并成功构建其原核表达载体pET-16b—SOD,为通过基因重组技术生产人SOD创造了条件。[著者文摘]
文章出处:
《动物医学进展》-2006年27卷12期 -91-94页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1007-5038(2006)12-0091-04
Cloning and Prokaryotic Expression of Human SOD Gene
SONG Kai ,SONG Ji-mei ( 1. Xuzhou Higher Vocational School of Bioengineering, Xuzhou, Jiangsu, 221006, China 2. Institure ofBiology Engineering ,Northwest A & F University ,Yangling ,Shaanxi,712100 ,China)
Abstract:
SOD is superoxide dismutase, it can clear away excessive O2^-· which is produced during the course of organism supersession. It can prevent various diseases and delay the decrepitude of human. We have accurately cloned human SOD gene fragment by RT-PCR and constructed the pET-16b-SOD vector. The cloning of human SOD gene and the construction of it 's expression vector lay a good foundation for further study on their function,biological activity and application.[著者文摘]
Key words:
human SOD; gene cloning; prokaryotic expression
基金资助:
国家“863”项目(2001AA213081)

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