学术
Dual effect of Molt-4 cell line onset with roscovitine
Haocheng Long Xia Gao Xiaolan Li Hui Xiao Xiaojun He Deding Tao Jianping Gong
Cancer Research Institute, Tongji hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China
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文章出处:
《中德临床肿瘤学杂志：英文版》-2007年6卷4期 -408-410页
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Dual effect of Molt-4 cell line onset with roscovitine
Haocheng Long, Xia Gao, Xiaolan Li, Hui Xiao, Xiaojun He, Deding Tao, Jianping Gong（ Cancer Research Institute, Tongji hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China）
Abstract:
Objective： To observe the effect of roscovitine （ROSC） as some Cyclin-dependent kinases （CDKs）, inhibitor onset with the Molt-4 cell lines. Methods： DNA assay of single cells by flow cytometry was used to detect the effect of cell cycle arrest and Annexin-V/FITC assay was used to detect the effect of cell apoptosis when the Molt-4 cell lines cultured with different concentration ROSC in different time points. Results： It showed that ROSC exerted strong inhibitory effect on proliferation and cell cycle progression of Molt-4 cell lines, accumulation of G2/M cells arrested took place after onset with 10 IJm and 20 μm ROSC more than 6 h; at the same time, the cell apoptosis of Molt-4 cells would be detected. According with the time and concentration increasing, the cell apoptosis rate would rise. Conclusion： It is concluded that Roscovitine （ROSC） as some Cyclin-dependent kinases （CDKs）, inhibitor, has dual effects to Molt-4 cells, not only the effect of cell cycle arrest but also the effect of cell apoptosis.[著者文摘]
Key words:
apoptosis; G2/M arrest; Molt-4 cells; FACS analysis
基金资助:
Supported by a grant from the National Nature Science Foundation of China （No. 30570908）.
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