学术
HPLC-ELSD法测定杏灵颗粒中萜类内酯的含量
马凤仙[1] 邱金东[2] 李钦[2]
[1]河南大学成人教育学院,河南开封475001 [2]河南大学中药研究所,河南开封475004
摘 要:
目的：测定杏灵颗粒中萜类内酯的含量。方法：采用高效液相色谱法蒸发光散射检测器（HPLC-ELSD）测定杏灵颗粒中萜类内酯的含量。色谱条件为：Angilent Hypersil ODS（5μm,4 mm×250 mm）柱;四氢呋喃-甲醇-水（10∶20∶70）为流动相;蒸发光散射检测器检测参数：漂移管温度103℃;载气（N2）流速2.51 L/min。结果：该方法简便易行,杏灵颗粒中基质对萜类内酯的测定无干扰,线形关系良好;萜类内酯的重现性试验RSD为1.63%;平均加样回收率为96.8%。结论：本方法可作为杏灵颗粒中萜类内酯成分质量控制的一种有效方法。[著者文摘]
文章出处:
《河南大学学报：医学版》-2007年26卷3期 -25-27页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1672-7606（2007）03-0025-03
Determination of terpenelactones in Xingling Granules by HPLC-ELSD
MA Feng-xian, QIU Jin-dong, LI Qin （ 1. School of Adult Education, Henan university, Henan Kaifeng 475001, China; 2. Institute of chinese Materia Medica, Henan university, Henan Kaifeng 475004, China）
Abstract:
Objective： To determine the content of terpenelactonesines in Xingling Granules. Methods： HPLC with Evaporative Light Scattering Detector（ELSD） was established to determine the content of terpenelactones in Xingling Granules. The analysis was carried out on Angilent Hypersil ODS（5 μm, 4 mm × 250 mm） column. The mobile phase consisted of THF-MeOH-H2O（ 10： 20： 70） . The operating conditions of the ELSD were a nebulizer nitrogen flow-rate of 2.51 L/min and an evaporator tube temperature of 103 ℃. Results： No interference was found in the terpenelactones peak position. The method was simple, with a good linear relationship. The relative standard deviation of reproducibility was 1.63 % and the average recovery was 96.8 %. Conclusion： The method can he used for quality control.[著者文摘]
Key words:
Xingling Granules; terpenelactones; HPLC; ELSD
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