学术
东俄洛橐吾遗传变异与分化的ISSR分析
王金凤[1,2] Chiaki kuroda[3] 龚洵[1]
[1]中国科学院昆明植物研究所,云南昆明650204 [2]中国科学院研究生院,北京100049 [3]Department of Chemistry, Rikkyo University, Tokyo 171-8501, Japan
摘 要:
应用ISSR标记对东俄洛橐吾（Ligularia tongolensis）的遗传多样性进行了研究。从100个引物中筛选出8个用于正式扩增。在所研究的8个居群共150个个体中检测到148个多态位点。在居群水平上,多态位点百分率（PPB）为50.45%,Nei′s基因多样性指数（H）和Shannon信息指数（I）分别为0.1595和0.2440。在物种水平上,多态位点百分率（PPB）为88.10%,Nei′s基因多样性指数（H）和Shannon信息指数（I）分别为0.2811和0.4279。居群间的遗传分化系数（Gst）达0.4355。研究结果表明东俄洛橐吾的遗传多样性水平很高,居群间遗传分化较大。这与其多样化的生态环境是有必然联系的。因适应其多样化的生态环境而形成了遗传多样性;且因其生态环境的不连续性阻碍了居群间的基因交流而产生了遗传分化,即东俄洛橐吾高水平的遗传多样性和遗传分化是适应其分布区多样化生态环境的结果。[著者文摘]
文章出处:
《云南植物研究》-2007年29卷5期 -537-542页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
0253-2700（2007）05-537-06
Assessment of Genetic Variation and Differentiation of Ligularia tongolensis （Compositae） Detected by ISSRs
WANG Jin-Feng, Chiaki kuroda, GONG Xun （1 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China ;2 Graduate School of the Chinese Academy of Sciences, Beijing 100049, China; 3 Department of Chemistry, Rikkyo University, Tokyo 171-8501, Japan）
Abstract:
The genetic diversity of Ligularia tongolensis （ Franch. ） Hand.-Mazz. was analyzed by inter-simple sequence repeat markers （ISSR）. Eight informative and reliable primers were chosen from 100 pre-screened primers, of which, 148 ISSR loci were polymorphic. Genetic diversity was high with PPB = 50.45%, H = 0. 1595 and 1 = 0.2440 at population level; PPB = 88.10%, H = 0.2811 and I = 0.4279 at species level. The G,, = 0.4355 indicated a high degree of genetic differentiation occurred among L. tongolensis populations. The main factor responsible for the high level genetic diversity and genetic differentiation among populations ascribed to diversified habitats diversification. Gene flow was inhibited by its habitats discontinuity which finally caused the genetic differentiation.[著者文摘]
Key words:
Ligularia tongolensis （Franch.） Hand.-Mazz. ; Population; Genetic diversity; Genetic differentiation
基金资助:
国家自然科学基金（30670210） 致谢 感谢本实验室的杨杨、杨淑达及潘跃芝在数据分析方面所给予的帮助.
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