学术
珍稀濒危植物安徽羽叶报春遗传多样性的RAPD分析
彭艳秋[1] 邵剑文[1,2] 张小平[1,2] 张中信[1] 朱国萍[1,2]
[1]安徽师范大学生命科学学院生物大分子进化重点实验室,安徽芜湖241000 [2]生物环境与生态安全安徽省高校重点实验室,安徽芜湖241000
摘 要:
利用RAPD分子标记对安徽特有濒危物种安徽羽叶报春（Primula merrilliana）6个自然居群的134个个体的遗传多样性进行了研究。从100个随机引物中筛选出12个RAPD引物,扩增共得到158条带,其中129个多态性位点（PPL）。POPGENE分析显示安徽羽叶报春具有较丰富的遗传变异（PPL=81.65%,He=0.2515,Ho=0.3849）。Nei′s基因多样性指数计算的居群间遗传分化系数（GST=0.5511）与Shannon信息指数（54.48%）基本一致。生境的片段化和基因流障碍可能是导致居群间遗传分化显著的主要原因。针对安徽羽叶报春的居群遗传变异提出了相应的保护措施：保护好自然生境和现有的居群及个体;加强居群间的基因流动;在迁地保护过程中,在尽可能多的居群中采样,以提高栽培居群的遗传多样性。[著者文摘]
文章出处:
《云南植物研究》-2007年29卷5期 -549-553页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
0253-2700（2007）05-549-05
Genetic Diversity of the Endangered and Endemic Species, Primula merrilliana （Primulaceae）, Revealed by RAPD Analysis
PENG Yan-Qiu, SHAO Jian-Wen, ZHANG Xiao-Ping, ZHANG Zhong-Xin , ZHU Guo-Ping （1 The Key Laboratory of Bio-Macromolecular Evolution, College of Life Science, Anhui Normal University, Wuhu 241000, China; 2 Anhui Key Laboratary of Biotic Environment and Ecological Security, Wuhu 241000, China）
Abstract:
Genetic diversity of 134 individuals from 6 natural populations of Primula merrilliana, an endangered and endemic species in Anhui province, was assessed using RAPD markers. A total of 158 amplified bands were evaluated from 12 informative and reliable primers screened from 100 RAPD primers, and 129 bands were polymorphic loci （PPL）. Analysis with POPGENE showed a high proportion of genetic variation （ PPL = 81.65%, He = 0.2515, Ho = 0.3849） for Primula merrilliana. Analyzed by Nei＇ s gene diversity index, the coefficient of the genetic differentiation （ Gsr ） was 0.5511, which was consistent with Shannon information index （54.48%）. The main factors responsible for the high degree of genetic differentiation among populations may result from habitat fragmentation and barriers of gene flow. The pattern of genetic variation provides important data and valuable information for the conservation strategies of Primula merriffiana, such as protecting their natural habitats and existing populations and individuals, improving the gene flow among populations, and sampling within more populations during ex situ conservation.[著者文摘]
Key words:
Primula merrilliana Schltr. ; RAPD; Genetic diversity; Genetic differentiation
基金资助:
教育部科学技术研究重点项目（206066）;安徽省优秀青年科技基金（06043089）;安徽省引进海外留学人才基金（2005殉32）：安徽省教育厅自然科学研究重点项目（2006KJ061A）资助 致谢 植物基因组DNA的提取工作是在浙江大学植物系统与进化重点实验室完成的,实验过程得到了浙江大学生命科学学院邱英雄博士和李恩香博士的帮助.
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