学术
中药含药血清对尿酸盐诱导的人血管内皮细胞ICAM-1表达的影响
徐红[1] 杨汝春[1] 洪华[2] 朱晓玲[1] 王军[1]
[1]浙江中医药大学附属广兴医院杭州市中医院,杭州310007 [2]金华市中医院,金华321000
摘 要:
目的：观察中药尿酸利仙（含药血清）对尿酸盐诱导的人血管内皮细胞（endothelial cell of vessels，ECV）细胞间黏附分子-1（intercelluar adhesion molecule，ICAM-1）基因和蛋白表达的影响。方法：1070μmol／L尿酸盐溶液刺激血管内皮细胞，同时用含不同浓度（2．5％、5．0％、10．0％）中药含药血清的培养液进行干预，分别用流式细胞术（FCM）、逆转录-聚合酶链式反应（RT—PCR）技术测定细胞ICAM-1的表达。结果：①血管内皮细胞在尿酸盐刺激下，ICAM-1基因和蛋白表达均较空白对照组显著上调。②5．0％浓度中药尿酸利仙含药血清可显著抑制尿酸盐诱导的血管内皮细胞ICAM-1基因表达（P〈0．01）；2．5％、10．0％浓度的尿酸利仙含药血清均可显著抑制ICAM-1蛋白表达（P〈0．01）。结论：中药尿酸利仙含药血清抑制ICAM-1表达的作用是减轻尿酸盐诱导的人血管内皮细胞炎症损伤的机制之一。[著者文摘]
文章出处:
《中华中医药杂志》-2007年22卷11期 -798-800页
栏目信息:
分 类 号:
Effects of Chinese materia medica contained serum on expression of ICAM-1 in Human Vascular Endothelial Cell induced by Urate
XU Hong, YANG Ru-chun, HONG Hua, ZHU Xiao-ling, WANG Jan, （IGuangxin Hospital Affiliated to Zhejiang University of TCM, Hangzhou TCM Hospital, Hangzhou 310007, China; 2jinhua TCM Hospital, Jinhua 321000,China）
Abstract:
Objective： To observe the effect of NiaoSuanLiXian on the expression of intercellular adhesion molecule （ICAM-1） in human vascular endothelial cells. Methods： 1070μmol/L urate solution was used to stimulate the vascular endothelial cells. At the same time, different levels of Chinese materia medica contained serum （2.5 %, 5%, 10%） were used to co-incubate cells. Flow cytometry and RT-PCR were used to detect the expression of ICAM-1 in the vascular endothelial cells. Results： Compared with control group, the mRNA and protein expression of ICAM-1 in the vascular endothelial cells induced by urate increased significantly. 5% of Chinese materia medica contained serum can down-regulate the mRNA expre＇ ssion of ICA M-1 significantly. 1% and 10% of Chinese mate- ria medica contained serum can down-regulate the protein expression of ICAM-1 remarkably. Conclusion： NiaoSuan- LiXian can reduce the inflammatory injure of human vascular endothelial cell induced by urate by down-regulating the expression of ICAM-1.[著者文摘]
Key words:
NiaoSuanLiXian; Intercellular adhesion molecule-1 ; Urate; Human vascular endothelial cell
基金资助:
浙江省自然科学基金资助项目（No.M303903）
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