学术
用新型分子模版吸附树脂从发酵液中分离红霉素的研究
鲁力[1] 王亚宁[2] 熊春华[3] 余蓉[1] 王槐三[2]
[1]四川大学华西药学院,成都610065 [2]四川大学国家生物医学材料工程中心,成都610065 [3]浙江工商大学化学系,杭州330306
摘 要:
采用新型分子模版吸附树脂动态吸附、丙酮解吸、水稀释结晶和丙酮重结晶等流程，从红色链霉菌发酵滤清液中分离红霉素，研究影响吸附分离效率的各种因素，并与现行醋酸丁酯萃取生产流程比较。结果显示，改性LX-18树脂对红霉素的吸附量达130ku／mL，对发酵液中色素的选择分离率〉95％，红霉素浓度120～150ku／mL的丙酮解吸液采用水稀释结晶，再用丙酮重结晶，即获得各项理化指标达药典标准的红霉素。结晶母液须2次吸附回收，红霉素总收率为60％～62％。本工艺具有简便、分离成本较低和减轻废水污染的特点。[著者文摘]
文章出处:
《四川农业大学学报》-2007年25卷3期 -328-331页
栏目信息:
文献标识码:
A
文章编号:
1000-2650（2007）03-0328-04
Study on the Separation Fermentation Liquid by of Erythromycin from New Absorption Resin
LU Li, WANG Ya-ning, XIONG Chun-hua, YU Rong, WANG Huai-san（ 1. West China School of Pharmacy Sichuan University, Chengdu 610065, China; 2. National Engineering Rmearch Center for Biomaterials Sichuan University, Chengdu 610065, Ch 3. Department of Chemistry, Zhejiang Gongshang University, Hangzhou 310035, China）
Abstract:
Useing neotype resin development-absorbing, acetone-desorption, water diluting to crystallize and acetone-recrystallisation separate the Erythromycin from red streptomycete fermentation scrubber liquid. Study all kinds of influence factors and compare with currently butylacetate-abstraction production flow-sheet. The result shows that the new reshaping resin LX- 18 does so well in absorbing the Erythromycin, its adsorption quantity reach 130 ku/mL and the separation for pigmentosus 95%, the Erythromycin concentration by 120N 150 ku/mL of acetone-desorption liquid use water diluting to crystallize, then acetone-recrystallisation. After that, it＇s all physical and chemical indicatrix can gain the pharmacopoeia standard. The crystal mother liquid need to twice adsorbing-recover, and the total yield of Erythromycin can reach 60 % -62 %. This separation method possesses simple process, high efficiency, low cost and only mild pollution wastewater.[著者文摘]
Key words:
Erythromycin; absorption resin; fermentation liquid
基金资助:
科技部科技型中小企业创新基金（06C26215100476）;浙江省科技厅科技计划资助项目（2005C33043）.
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