学术
体内和体外法比较保护蛋氨酸和赖氨酸产品在瘤胃和小肠的释放率
郭玉琴[1,2] 王加启[1] 卜登攀[1] 魏宏阳[1] 周凌云[1]
[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100094 [2]北京农学院动物科学技术系,北京102206
摘 要:
采用不同材料制得6种保护蛋氨酸和4种保护赖氨酸产品,蛋氨酸分别以丙烯酸树脂Ⅳ号（M1）,丙烯酸树脂Ⅳ号和乙基纤维素（M2）,丙烯酸树脂Ⅳ号和乙基纤维素及羟丙基甲基纤维素（M3）,乙基纤维素（M4）,丙烯酸树脂Ⅳ号和氢化植物油（M5）,氢化植物油（M6）包被;赖氨酸分别以丙烯酸树脂Ⅳ号和乙基纤维素（L1）,丙烯酸树脂Ⅳ号和羟丙基甲基纤维素（L2）,丙烯酸树脂Ⅳ号和氢化植物油（L3）,乙基纤维素和氢化植物油（L4）包被。对10种产品进行了体外和尼龙袋法稳定性检验。结果表明,用尼龙袋在4头荷斯坦奶牛瘤胃中培养4、8、24、48 h,丙烯酸树脂Ⅳ号和氢化植物油保护的M5和L3的释放率最低,分别为11.72%-50.67%、35.70%-72.09%;其在pH6.6缓冲溶液中培养相应时间的释放率分别为24.67%-38.44%,42.05%-75.02%,体外法与尼龙袋法测定结果显著相关,相关系数分别为RM^2=0.867 5,RL^2=0.963 0。M5和L3在真胃和小肠及在pH2.4缓冲溶液中的释放率均为100%。[著者文摘]
文章出处:
《畜牧兽医学报》-2007年38卷11期 -1257-1261页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
0366-6964（2007）11-1257-05
Ruminal Degradation and Intestinal Release Rate of Protected Methionine and Lysine
GUO Yu-qin ,WANG Jia-qi ,BU Deng-pan ,WEI Hong-yang ,ZHOU Ling-yun （1. State Key Laboratory of Animal Nutrition , Institute of Animal Sciences, Chinese Academy of Agricultural Sciences ,Beijing 100094, China ; 2 . Department of Animal Science and Technology ,Beijing Agricultural College ,Beijing 102206, China）
Abstract:
Rumen-protected methionines （RPMet） were obtained from polyoxylate Ⅳ （M1）, polyoxylate Ⅳ and ethyl-cellulose （M2）, polyoxylate Ⅳ and ethyl-cellulose and hydroxyprpoyl methylcellulose（M3）, ethyl-cellulose（M4）, polyoxylate Ⅳ and hydrogenated vegetable oil（M5）, and hydrogenated vegetable tallow （M6）. Rumen-protected lysines （RPLys） were obtained with polyoxylate Ⅳ and ethyl-cellulose（L1）, polyoxylate Ⅳ and hydroxyprpoyl methylcellulose（L2）, polyoxylate Ⅳ and hydrogenated vegetable oil（L3）, ethyl-cellulose and hydrogenated vegetable oil （L4）. The stability of RPMet and RPLys was tested by in vitro and in situ. After incubating for 4, 8, 24 and 48 h in the rumen ,the ruminal release rate of nitrogen was lower and ranged from 11.72% -50.67% and 35. 70%-72. 09% inM5 and L3, respectively. However ,when incubated in the buffer solution（pH= 6.6） for 4, 8, 24 and 48 h, nitrogen release rate ranged from 24.67%-38.44% and 42. 05%- 75. 02% in M5 and L3, respectively. There was a significant positive linear relationship （RM^2 = 0. 867 5,RL^2 = 0. 963 0） of the degradability of RPMet and RPLys between in vitro and in situ. Nitrogen release rate was 100% in M5 and L3 in the small testine and the buffer solution（pH=2.4）.[著者文摘]
Key words:
methionine; lysine; rumen; small intestine ;release rate; in situ; in vitro
基金资助:
国家科技支撑计划项目（2006BAD04A03;2006BA12B08）
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