学术
摘 要:
用毛白杨中克隆得到的抗坏血酸过氧化物酶（APX）基因,构建植物表达载体pBI121-APX,将pBI121-APX载体用农杆菌介导法转化烟草,成功得到转基因烟.PCR、PCR-Southern的结果表明,APX基因已经成功的整合到转基因烟草基因组中.干旱实验结果表明,与对照相比较,转基因烟草表现出较强的抗旱性.[著者文摘]
文章出处:
《成都大学学报：自然科学版》-2007年26卷2期,121 -93-96,121页
分 类 号:
文献标识码:
A
文章编号:
1004-5422（2007）02-0093-04
Research of Transgenic Tobacco of APX Gene in Drought Tolerance Analysis
HAN Ruili, LU Hal （College of Biological Science and Biotechnology, Beij ng Forestry University, Beijing 100083, China）
Abstract:
In this paper, the APX gene cloned from Populus tomentosa was used for the construction of an expression vector： pBI121-APX, it was used for the genetic transformation of tobacco via Agrobacterium mediated method. PCR and PCR-Southem blot analysis indicated that the foreign gene APX had been integrated into the genome of tobacco. Transgenic tobacco showed more resistance under drought stress.[著者文摘]
Key words:
ascorbate peroxidase; vector construction; drought resistance; transgenic tobacco
基金资助:
教育部科学技术研究重点项目（106039）,北京材业大学研究生自选课题（05jj039）资助
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