学术
ERK1／2信号转导通路对骨髓基质细胞条件培养液诱导大鼠中脑神经干细胞分化作用的影响
顾平[1,2] 王彦永[1,2] 马芹颖[1,2] 温雅[1,2] 崔冬生[1] 刘力[1] 王铭维[1,2] 张振清[1,2]
[1]河北省脑老化与认知神经科学实验室,石家庄050031 [2]河北医科大学第一医院神经内科,石家庄050031
摘 要:
目的：探讨ERKl／2信号转导通路在骨髓基质细胞条件培养液（BMSCs-CM）诱导大鼠中脑神经干细胞（NSCs）分化中的作用。方法：将神经干细胞球种植于预先包被多聚赖氨酸的35mm的培养皿中，神经干细胞球约30min后贴壁，自然分化组将培养液换为含2％B27的neurobasal培养液，对照组换为BMSCs-CM，抑制剂组将培养液换为含有信号转导通路抑制剂PD98059（5μmol／L）的BMSCs-CM，7d后采用免疫荧光染色方法观察NSCs后代细胞中神经元和星形胶质细胞的数量比例与形态差别。结果：用成年大鼠BMSCs-CM体外培养新生大鼠中脑NSCs，自然分化组NSCs分化的神经元为（15．7±10．3）％[低于对照组的（51．17±10．30）％，P〈0.05],星形胶质细胞为（66．9±14.8）％[明显高于对照组的（23．26±5．60）％，P〈O．05]。而抑制剂组神经元所占比例为（39．48±7．29）％[明显低于对照组，P％0．01]，星形胶质细胞的比例为（31．01±8．96）％[明显高于对照组，P％0．01]。结论：BMSCs-CM可以提高神经干细胞分化为神经元的比例，并且同时降低了其分化为星形胶质细胞的比例，这两种作用很可能是通过ERKl／2信号转导通路实现的。[著者文摘]
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文章出处:
《第二军医大学学报》-2007年28卷6期 -589-592页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
0258-879X（2007）060589-04
Influence of ERK1/2 signal-transduction pathway on differentiation of mesencephalic neural stem cells induced by bone marrow stromal cell-conditioned medium
GU Ping,WANG Yan-yong,MA Qin-ying,WEN Ya, CUI Dong-sheng,LIU Li,WANG Ming wei,ZHANG Zhen- qing（1. Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province, Shijiazhuang 050031, China; 2. Department of Neurology, the First Affiliated Hospital of Hebei Medical University, Shijiazhuang 050031）
Abstract:
Objective：To investigate the effect of the ERK1/2 signal-transduction pathway on differentiation of rat mesencephalic neural stem eells（NSCs） in presence of bone marrow stromal cell-conditioned medium（BMSCs-CM）in vitro. Methods： NSCs were cultured on poly-L-lysine （PLL） coated 35 mm dishes. The neurospheres adhered to the bottom 30 min later; then the culture medium of natural differentiation group was replaced by neurobasal medium containing 2% B27; the control group was replaced with BMSCs-CM; and the inhibition group was replaced with BMSCs-CM containing PD98059 （5 μmol/L）. The proportions of neurons and astroeytes and their morphological difference were observed by immunocytochemistry technique 7 d later. Results： We successfully cultured NSCs from adult rat BMSCs-CM. The proportion of neurons in the natural differentiation group （15.70±10.3）% was lower than that in the control group （[51.174±10.30]%, P〈0.05） ;the proportion of astrocytes in the natural differentiation group （66.94±14.8）% was higher than that in the control group （E23.26±5.60]%/,P〈0.05）. The proportion of neurons in the inhibition group （39.48±7.29）% was lower than that in the control group （[51.17±10.30] %, P〈0.05）; the proportion of astrocytes in the inhibition group （31.01±8.96）% was higher than that in the control group （[23.26±5.60]% ,P〈0.01）. Conclusion： BMSCs-CM can up-regulate the proportion of neurons in cultured NSCs and down-regulate the proportion of astrocytes, which might be mediated by the ERK1/2 signal-transduction pathway.[著者文摘]
Key words:
ERK1/2 signal-transduction pathway; bone marrow stromal cells; conditioned medium; neural stem cells
基金资助:
基金项目：国家自然科学基金（30400138）;河北省自然科学基金（C2004000583）.
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