向日葵菌核病生防放线菌的分离筛选及拮抗作用的初探
胡俊[1] 刘正坪[2] 周洪友[1] 丁俊[1] 宋娟[1]
[1]内蒙古农业大学农学院,内蒙古呼和浩特010019 [2]北京农学院植物科学技术系,北京102206
摘 要:
根据微生物间的拈抗作用,试从病地土壤中分离拮抗放线菌,探索利用放线菌对向日葵菌核病进行生物防治的途径。从内蒙古呼和浩特市、巴彦淖尔、包头等地所采集的土壤中分离出38个放线菌菌株,经过平板拮抗作用初测,有8个菌株(F-1、F-3、F-6、F-8、F-11、F-15、F-21、F-25)对向日葵核盘菌(Sclerotinia sclerotiorum)有拮抗作用。选出其中2个作用最明显的菌株(F-6、F-8),观察其在平板对峙培养中对向日葵核盘菌的抑制作用及其代谢产物对向日葵核盘菌的作用。结果表明:F-6、F-8菌株及其代谢产物对向日葵核盘菌均有明显的抑菌作用。同时,对F-6、F-8两菌株的培养性状和形态特征进行了初步观察。[著者文摘]

文章出处:
《华北农学报》-2006年21卷1期 -96-99页
分 类 号:
文献标识码:
A
文章编号:
1000-7091(2006)01-0096-04
Studies on Isolation and Screening of Antagonisticmycetes-fungus and Its Antagonistic Effect for Sunflower Sclerotinia Rot
HU Jun, LIU Zheng-ping, ZHOU Hong-you, DING Jun, SONG Juan (1. Inner Mongolic Agricultural University, Huhhot 010019, China; 2. Beijing Agricultural College, Beijing 102206, China)
Abstract:
We explored the way of biological control by isolating antagonisticmycetes-fungus from pathogenic soil for Sclerotinia sclerotiorum. The result showed that 38 strains were isolated from soil samples derived from Huhhot, Bayannaoer and Baotou regions of Inner Mongolia. We observed that 8 strains, named as F - 1, F - 3, F - 6, F - 8, F - 11, F - 15, F - 21, F - 25, displayed antagonisms against S. sclerotiorum on the PDA plate. F- 6 and F - 8 were selected to study their inhibition to S. sclerotiorum growth and effects of their metabolic products on S. sclerotiorum of sunflower. Meanwhile, we examined the cultural and morphological characteristics of F - 6 and F - 8. Results showed that F - 6, F - 8 and their metabolic products could inhibit S. sclerotiorum growth on the PDA plate.[著者文摘]
Key words:
Sunflower; Sclerotinia sclerotiorum; Antagonisticmycetes-fungus; Metabolic products

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