学术
由甲壳素制备N-乙酰葡萄糖胺二聚糖的研究
吴平格[1] 高辉[2] 王宏雁[1] 黄雪[1] 张鹏举[1]
[1]河南工业大学化学化工学院,河南郑州450052 [2]洛阳大学环境与化学工程学院,河南洛阳471000
摘 要:
虾壳经酸碱两次交替处理制得甲壳素．制得的甲壳素用浓盐酸部分水解得到N-乙酰葡萄糖胺低聚糖混合物，柱层析分离制备N-乙酰葡萄糖胺二聚糖（NAG）．试验考查了水解温度、水解时间、盐酸浓度对水解反应的影响以及脱洗液浓度与二聚糖纯度的关系．甲壳素水解制备N-乙酰葡萄糖胺二聚糖的适宜反应条件为水解温度50℃，水解时间5、5h，盐酸浓度6．0mol／L．分离N-乙酰葡萄糖胺二聚糖的最佳脱洗液浓度为5％乙醇溶液，此条件下N-乙酰葡萄糖胺二聚糖的得率最高可达24．06％．[著者文摘]
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文章出处:
《河南科学》-2005年23卷5期 -663-666页
分 类 号:
文献标识码:
A
文章编号:
1004-3918（2005）05-0663-04
The preparation of dimeric N-acet-glucosamine by hydrolytic degradaion using hydrochloric acid from chitin
Wu PingGe;Gao Hui;Wang HongYan;Huang Xue;Zhang PengJu
Abstract:
Chitin was obtained by alternately dealing shrimp-shells with acid and alkali. By partial hydrolysis of chitin in concentrated hydrochloric acid to get the mixture of chitodigosacharides, the outcomes were chromatographed on charcoal column for purification, then dimeric N-acet-glucosamine was obtained. The experiment investigated infections of temperature and time as well as the concentration of experiments hydrochloric to the partial hydrolysis of chitin. The results of experiments suggest that the best conditions of chitin＇ s hydrolysis were 50℃/5.5h/6.0mol·L^-1 hydrochloric acid. The best concentration of washing ,solution to separate N-acet-glucosamine was 5 % ethanal solution. On these conditions, the dimeric N-acet-glucosamine was accessible in the highest yield 24.06 %.
Key words:
shrimp-shells; chitin; dimeric N-acet-glucosamine
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