学术
茶皂素杀线虫活性测定分析
吴慧平[1] 宛晓春[1] 侯如燕[1] 徐晓莉[2]
[1]安徽农业大学农业部／教育部茶叶生物化学与生物技术重点实验室,合肥230036 [2]安徽农业大学植物保护学院,合肥230036
摘 要:
茶皂素（Tea saponin，TS）是由各类茶籽（油茶Thea sinesis或茶Camellia sasauqua）饼粕中提取出来的五环三萜类化合物，具表面活性、生理活性和鱼毒活性以及溶血性等。近10年来，国内有关TS杀虫抑菌活性有较多的研究报道，但未见杀线虫活性方面的报道。植物线虫在生产上的危害逐年加重，化学杀线剂价格昂贵、毒性大、残留大，生产中亟待寻找替代品控制线虫病害。TS是生物副产品，我国每年至少有100万t油茶饼产生，可产生30万t以上的茶皂素。基于如上考虑，作者对TS杀线虫活性进行了初步的测定分析。[第一段]
文章出处:
《植物病理学报》-2007年37卷5期 -553-555页
栏目信息:
分 类 号:
文章编号:
0412-0914（2007）05-0553-03
Bioassay of nematicidal activity of Tea saponin
WU Hui-ping, WAN Xiao-chun, HOU Ru-yan , XU Xiao-li （1 Key Laboratory of Tea Biochemistry ＆ Biotechnology of Ministry of Agriculture, Anhui Agricultural University, Hefei 230036, China; 2School of Plant Protection, AAU, Hefei 230036, China）
Abstract:
In order to determine the nematicidal activity of Tea saponin （TS）, the oil tea cakes were processed through degreasing , dealcholizing, and extracting with n-butyl alcohol, the crude powder of 84.6% TS was obtained. The TS powder was evaluated against 4 plant nematodes and 1 soil nematode. The results indicated that TS exhibited modest nematicidal activity against the 4 plant nematodes, but exhibited no nematicidal activity against Rhbiditis sp.. The pot experiments showed that infective inhibition of TS against Hrischamanniells oryzae is 71.1%, galls on Trichosanthes kirilowii caused by Meloidogyne incognita was reduced by 88.9% when the bare-root-tip was placed in TS solution for 3 h.[著者文摘]
Key words:
Tea saponin; plant parasitic nematode; nematicidal activity
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