学术
内蒙古西部地区砂珍棘豆（Oxytropis racemosa Turcz．）遗传多样性分析
卢萍[1,2] 赵萌莉[1] 韩国栋[1] 曹珍[2] 陈曦[2] 王中南[2]
[1]内蒙古农业大学生态环境学院,呼和浩特010018 [2]内蒙古师范大学生命科学与技术学院,呼和浩特010022
摘 要:
用ISSR分子标记对内蒙古西部地区砂珍棘豆（Oxytropis racemosa Turcz．）3个不同生境的地理种群进行了种群遗传多样性分析．结果表明：砂珍棘豆种群具有较高的遗传多样性，14个ISSR引物扩增出284条带，Shannon多样性指数，一0．3630，Nei’s多样性指数H=0．2234，物种总基因多样性（HT）为0．2236，种群内基因多样性（Hs）为0．2085，大部分遗传变异（93．27％）存在于种群内，6．73％的遗传变异存在于种群间．遗传分化系数（Gst）为0．0673，基因流（Nm）为6．9318，说明种群间有很强的基因交流，遗传漂变不会引起遗传分化．UPGMA遗传距离聚类结果表明，3个地理种群中，乌审旗和伊金霍洛种群先聚在一起，然后与鄂托克种群聚在一起，遗传距离与地理距离相关，[著者文摘]
文章出处:
《内蒙古大学学报：自然科学版》-2007年38卷2期 -160-165页
分 类 号:
文献标识码:
A
文章编号:
1000-1638（2007）02-0160-06
Population Genetic Diversity Analysis of Oxytropis racemosa Turcz. in Inner Mongolia Western Regions
LU Ping ,ZHAO Meng-li ,HAN Guo-dong ,CAO Zhen ,CHEN Xi ,WANG Zhong-nan （1. College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Hohhot 010018, China 2. College of Lifc Science and Technology, Inner Mongolia Normal University ,Hohhot 010022,China）
Abstract:
The genetic diversity of Oxytropis racemosa Turcz. was analyzed using inter simple sequence repeat （ISSR） markers. The results indicated that there was relatively high genetic diversity in O. racemosa Turcz in Inner Mongolia western regions. 284 bands were amplified from 3 populations in different habitats with 14 reliable primers. The Shannon information index I was 0. 3630, and Nei＇s index H was 0. 2234. The total gene diversity Ht was 0. 2236, and the gene diversity within populations Hs was 0. 2085. 93.27% of genetic variation existed within populations while 6.73% of genetic variation existed among populations. The Gst was 0. 0673,and the gene flow was 6. 9318. There was a strong gene flow among the populations. So the genetic drift would not cause the genetic differentiation. The UPGMA Dendrogram displayed that the Wushen and Yijinhuoluo populations clustered first, and then they clustered with the Etoke population. The results showed the genetic distance related to geographic distance in three populations of Oxytropis racemosa Turcz.[著者文摘]
Key words:
Oxytropis racemosa Turcz. ;ISSR;genetic diversity;Nei＇s index ;Shannon index
基金资助:
国家自然科学基金（30360022）、内蒙古自然科学基金（200607010504）项目资助;致谢：衷心感谢内蒙古师范大学金凤讲师、内蒙古农业大学硕士研究生杨晓慧、牛海、韩雄,博士研究生王晓宇、王雨梅及鄂尔多斯地方草原站的同志们在野外采样和DNA提取工作中的大力帮助.
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