学术
摘 要:
目的探讨主要组织相容性复合物（major histocompatmility complex，MHC）Ⅱ类反式激活因子（classⅡtransactivator，CⅡTA）在调控HepG2细胞HLAⅡ类分子表达中的作用。方法将含CⅡTAcDNA的真核表达载体EBS-NPL-CⅡTA和不含CⅡTA cDNA的空载体EBS-NPL分别转染至HepG2细胞。用RT-PCR技术检测未经转染的HepG2细胞、转染EBS-NPL-CⅡTA及空载体EBS-NPL的HepG2细胞CⅡTA mRNA的表达，并用间接细胞免疫荧光技术及流式细胞技术检测其3种HLAⅡ类分子（HLA-DR、DP、DQ）的表达。结果未经转染的HepG2细胞和转染空载体EBS-NPL的HepG2细胞均无CⅡTA mRNA和HLAⅡ类分子的表达。转染EBS-NPL-CⅡTA后的HepG2细胞出现CⅡTA mRNA表达，并表达3种HLAⅡ类分子。结论CⅡTA是调控HepG2细胞是否表达HLAⅡ类分子的关键因子，HepG2细胞不表达HLA-Ⅱ类分子与其缺乏CⅡTA表达有关，为进一步研究CⅡTA在肝癌治疗中的作用奠定基础。[著者文摘]
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文章出处:
《第三军医大学学报》-2007年29卷21期 -2019-2022页
栏目信息:
文献标识码:
A
文章编号:
1000-5404（2007）21-2019-04
Role of MHC class Ⅱ transactivator in upregulating the expression of HLA class Ⅱ antigen in HepG2 cells
BAI Xiu-juan, ZHANG Xu-qing （ Institute of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China）
Abstract:
Objective To investigate the role of MHC class Ⅱ transactivator （ C Ⅱ TA） in upregulating the expression of HLA class Ⅱ antigen in HepG2 cells. Methods Eukaryotic expression vector EBS-NPL-C Ⅱ TA containing C Ⅱ TA cDNA or the empty vector EBS-NPL was transfected into HepG2 cells respectively. C Ⅱ TA mRNA and HLA class Ⅱ antigen （HLA-DR, DP, DQ） were respectively detected by RT-PCR, indirect cell immunofluorescence technique and flow cytometry in original HepG2 cells, HepG2 cells transfected with EBS- NPL-C Ⅱ TA or the empty vector EBS-NPL. Results The expression of C Ⅱ TA mRNA and HLA class Ⅱ antigen （HLA-DR, DP, DQ） were not observed in original HepG2 cells and HepG2 cells transfected with empty vector, but in the HepG2 cells transfected with EBS-NPL-C Ⅱ TA. Conclusion C Ⅱ TA is a switching factor of mastering the expression of HLA class Ⅱ antigen in HepG2 cells. The lack of C Ⅱ TA expression in HepG2 cells contributes to no expression of HLA Ⅱ antigen.[著者文摘]
Key words:
class Ⅱ transactivator; HepG2 cells; HLA class Ⅱ antigen
基金资助:
国家自然科学基金（30371283）
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