学术
细菌Sh34的鉴定及其对几种植物病原真菌的拮抗作用测定
董红强[1,2] 慕卫[1] 刘峰[1] 潘金菊[1] 翟茹环[1]
[1]塔里木大学植物科技学院,新疆阿拉尔843300 [2]山东农业大学植物保护学院,山东泰安271018
摘 要:
从黄瓜田土壤中分离得到拈抗细菌Sh34，根据其形态特征和生理生化特性及其细菌的16SrDNA序列分析，鉴定其为枯草芽孢杆菌（Bacillus subtil＆）。经平皿试表明，菌株sh34及其无菌滤液对黄瓜蔓枯病菌（Ascochytacucumis）、黄瓜枯萎菌（Fusariumoxysporum fisp．cucumerinum）、黄瓜菌核（（Sclerotiniascleotiorum）、瓜果腐霉菌（Pythium aphanidermatum）、立枯丝核菌（Rhixoctoniasolani）等多种植物病原真菌均有良好稳定的抑制作用。其无菌滤液能使黄瓜枯萎菌菌丝老化，原生质浓缩。[著者文摘]
文章出处:
《农药》-2007年46卷11期 -779-780页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1006-0413（2007）11-0779-02
Identification of Bacterium Sh34 and Measure of Its Inhibition to Some Pathogenic Fungi
DONG Hong-qiang, MU Wei, LIU Feng, PAN Jin-ju, ZHAI Ru-huan （l.lnstitute of Plant Science and Technology, Tarim University, Alaer 843300, Xinjiang, China; 2.College of Plant Protection, Shandong Agricultural University, Taian 271018, Shandong, China）
Abstract:
The antagonistic bacterial strain Sh34 isolated and screened from field soils of cucumber was identified asBucillus subtilis according to its morphological biochemical characters and analysis of the partial sequence of the16SrDNA. Both the bacterial cultural and aseptic filtrate showed activities to inhibit some pathogenic fungi including Ascochyta cucumis, Fusarium oxysporum f.sp.cucumerinum, Sclerotinia scleotiorum, Pythium aphanidermatum, Rhixoctonia solani on activity plates assay. Its aseptic filtrate is capable to accelerate the hypha to grow older and cytoplasm condensation of Fusarium oxysporum f.sp..[著者文摘]
Key words:
bacterium; identification; Bacillus amyloliquefaciens; antagonism
基金资助:
山东农业大学青年科技项目（200513）
更多>>免费文章
cqvip.com