学术
应激对Wistar大鼠牙周炎动物模型的影响
李博[1] 林崇韬[1] 周春华[2]
[1]吉林大学口腔医院牙周病科,吉林长春130041 [2]吉林大学口腔医院儿童牙科,吉林长春130041
摘 要:
目的：建立合适的牙周炎动物模型,探讨应激与牙周炎之间的关系。方法：40只Wistar大鼠用尼龙丝线结扎左上颌第二磨牙,随机分为应激组（给予限制性应激）和对照组。于第2、4、6、8和10天分批处死大鼠,检测应激指标血糖、促肾上腺皮质激素（ACTH）、皮质类固醇、肾上腺素含量,测量胸腺和脾脏重量与体重的相对比值。观察上颌第二磨牙根分叉处的组织学改变,测量根分叉区牙槽嵴高度与根分叉高度比值。结果：胸腺/体重、脾脏/体重、血糖、ACTH、皮质类固醇、肾上腺素在应激组与对照组间比较差异均有显著性（P〈0.05或P〈0.01）;应激组结扎区根分叉区牙槽嵴顶至根分叉距离与根分叉高度比在第8和10天显著低于对照组（P〈0.05或P〈0.01）。结论：Wistar大鼠适于建立应激牙周炎动物模型,限制性应激不会导致牙周炎的发生,但可以加重牙周组织炎症的破坏程度。[著者文摘]
文章出处:
《吉林大学学报：医学版》-2007年33卷5期 -856-859页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1671-587X（2007）05-0856-04
Effect of restraint stress on experimental periodontitis in Wistar rats
LI Bo , LIN Chong-tao , ZHOU Chun-hua （1. Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130041, China; 2. Department of Pediatric Dentistry, Stomatology Hospital, Jilin University, Changchun 130041, China）
Abstract:
Objective To establish a suitable animal model of experimental periodontitis and study the relationship between stress and inflammatory periodontal diseases. Methods Forty Wistar rats with nylon thread placed around the neck of maxillary left second molar tooth were divided randomly into two groups： stress group （the rats were treated with restraint strees for 12 h every day for 10 d） and control group. Four rats of either group were sacrificed at the day of 2, 4, 6, 8, 10, separately; the level of blood glucose and the contents of adrenocorticotropic hormone （ACTH）, orticosterone and adrenaline were measured as the stress markers, as well as the relative weight of the thymus and spleen. The furcation area of the second maxillary molar was examined histologically and histometrically. Results In stress group, all the markers for stress were significant higher than those in control group, and the thymus and spleen were atrophied （P〈 0.05 or P〈0.01）. Marked alveolar bone resorption occurred in stress group on the 8th and 10th day （P〈0.05or P〈0.01）. Conclusfon Wistar rat is suitable for establishing the animal model of experimental periodontitis. Restraint stress itself doesn＇t result in periodontitis whereas it modulates the progression of periodontitis.[著者文摘]
Key words:
restraint stress; periodontitis; rats, Wistar
基金资助:
吉林省科技厅科技发展计划项目基础自然科学基金资助课题（20020658）;吉林大学创新基金资助课题（419070200082）
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