学术
国际标准刊号：ISSN 1007-2764
国内统一刊号：CN 44-1620
摘 要:
对茂原轮枝链霉菌（Streptoverticilluim mobaraense）的原生质体制备进行了研究。研究表明在培养基中加入4%的甘氨酸有利于原生质体的释放。茂原轮枝链霉菌孢子悬液在41℃下用2．5mg／mL溶菌酶作用4h的效果最好，原生质体形成率与再生率的乘积可达24％。[著者文摘]
文章出处:
《现代食品科技》-2008年24卷2期 -138-142页
Modern Food Science and Technology
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1673-9078（2008）02-0138-05
相关文章:
Optimization of Protoplast Preparation from Spores of Streptoverticilluim mobaraense
BAO Ying-ling, PAN Li （College of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510640, China）
Abstract:
The preparations of protoplast of Streptoverticilluim mobaraense were studied. Results showed that 4% glycine containd in culture medium were propitious to release of protolast. The optimal preparation conditions were as follows： enzymatic temperature 41 ℃, lysozyme2.5 mg/mL, enzymatic time 4 h. Under those conditions, the product of the generation rate and regeneration rate reached 24%.[著者文摘]
Key words:
Streptoverticilluim mobaraense; Protoplast; Response surface methodology （RSM）
收稿日期: 2007-10-15
基金资助:
广东省科技攻关项目（2006B13001006）
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