学术
SLAⅠ类基因研究新进展
陶璇[1,2] 李华[1,2] 李学伟[2] 于辉[1] 左启祯[1]
[1]佛山科学技术学院动科系,南海528231 [2]四川农业大学动物科技学院,雅安625014
摘 要:
SLAⅠ类基因编码细胞表面高度多态的糖蛋白，负责将内源性抗原肽递呈给T细胞，激发特异的免疫反应。文章介绍了近年来SLAⅠ类基因分子结构、组织中的表达、表达调节、基因分型、多态性及进化研究的新进展，重点介绍了SLAⅠ类基因分型方法及多态性研究。SLAⅠ类基因分型方法主要包括血清学法、DNA序列测定、PCR—SSP、PCR—SSOP、微卫星法，其中PCR-SSP法快速简便，在SLAⅠ类基因分型研究中应用广泛。文章对SLAⅠ类基因在基因功能、肽疫苗、异种移植等方面的研究和应用前景进行了讨论。[著者文摘]
文章出处:
《遗传》-2007年29卷11期 -1309-1316页
栏目信息:
分 类 号:
文章编号:
10.1360/yc-007-1309
Latest advances of SLA class Ⅰ genes
TAO Xuan, LI Hua, LI Xue-Wei, YU Hui, ZUO Qi-Zhen（ 1. Department of Animal Science, Foshan University, Nanhai 528231, China; 2. College of Animal Science and Technology, Sichuan Agriculture University, Ya＇an 625014, China）
Abstract:
The Swine leukocyte antigen （SLA） class Ⅰ genes encode multi-glycoproteins on cell surface, which present endogenous antigenic peptides to T cells and thus initiate specific immune responses. In this article, latest advances on molecular structure, expression in tissues, regulation of expression, genotyping, polymorphism, and evolution of SLA class Ⅰ genes were introduced, in which genotyping and polymorphism were emphasized. Molecular typing methods of SLA class Ⅰ genes include serological method, DNA sequencing, PCR-SSP, PCR-SSOP and MS, of which PCR-SSP is frequently used in genotyping of SLA class Ⅰ genes as a simple and rapid method. Future directions for the study and application of SLA class Ⅰ genes on gene functions, peptide vaccine, xenotransplantation were also discussed.[著者文摘]
Key words:
SLA class Ⅰ gene; expression; polymorphism; evolution
基金资助:
广东省自然科学基金项目（编号：7010422）,广东省科技攻关项目（编号：20078020706001）和国家自然科学基金项目（编号：30671500）资助
更多>>免费文章
cqvip.com