学术
富血小板血浆对犬骨髓间充质细胞矿化能力的影响
潘永海[1] 李宁毅[2] 邱建忠[3] 金晓明[2] 陈立强[2] 刘相萍[4] 隋爱华[4]
[1]青岛大学医学院二附院,青岛市中心医院口腔颌面外科,青岛266042 [2]青岛大学医学院附属医院口腔颌面外科,青岛266003 [3]青岛大学医学院附属市立医院口腔颌面外科,青岛266031 [4]青岛大学医学院附属医院分子生物实验室,青岛266003
摘 要:
目的 研究富血小板血浆（platelet-rich plasma，PRP）对犬骨髓间充质细胞（marrow stromal cells，MSCs）碱性磷酸酶（alka-line phosphatase，ALP）及矿化结节形成能力的影响。方法 将不同浓度（10％、20％、30％）的PRP及重组人骨形成蛋白（rhBMP-2）加入体外培养的第3代MSCs中，对照组为无血清培养液，检测其对细胞ALP活性的影响；将第3代的MSCs在体外进行培养，实验组加入矿化液和PRP（10％或30％），对照组只加矿化液。4周后用茜素红及Von—Kossa染色检测矿化结节的形成。结果 10％PRP和rhBMP-2能显著提高MSCs的ALP活性，其中10％PRP对体外矿化结节的形成，亦有一定程度的促进作用。结论 PRP有促进MSCs向成骨细胞方向转化的趋势。[著者文摘]
文章出处:
《口腔医学》-2007年27卷5期 -232-235页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1003-9872（2007）05-0232-04
Effects of platelet-rich plasma on mineral characteristics of bone marrow stromal cells
PAN Yong-hai, LI Ning-yi, QIU Jian-zhong, JIN Xiao-ming, CHEN Li-qiang, LIU Xiang-ping, SUI Ai-hua. （ Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Medical College, Qingdao University; Qingdao Central Hospital, Qingdao 266042, China）
Abstract:
Objective To investigate the effects of platelet-rich plasma（PRP） on the alkaline phosphatase（ALP） activity and mineralized nodule formation of dog＇s bone marrow stromal cells（MSCs）. Methods Various concentrations of PRP（0,10%, 20%, 30% ） and rhBMP-2 were added to the medium of cultured third passage MSCs. After 3,6,9,12 days of co-incubation, the ALP activity was measured. After the third passage MSCs was cultured for 4 weeks, mineralized solution and PRP（ 10% ,30% ） were added into the test group medium. Mineralized solution was added into the medium of the control group. At day 28,the formation of mineralized nodules was detected with Dahl McGec-Russll＇ s alizarin red stain and yon Kossa＇ s silver nitrate for calcium. Results PRP at the concentration of 10% could increase ALP activity; the 10%PRP had an optimal effect（ P 〈 0.05）.PRP could also induce nodule formation to some degree. Conclusion PRP has the tendency of inducing MSCs to differentiate into osteoblastic cells.[著者文摘]
Key words:
platelet-rich plasma; bone marrow stromal cells; alkaline phosphatase; mineralization; dogs
基金资助:
国家自然科学基金资助项目（30471899）
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