学术
HPLC测定天然食品保鲜剂中丹参酮ⅡA含量
徐晓霞[1] 梁琪[1] 毕阳[1] 史彦斌[2] 王慧娟[1]
[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070 [2]兰州大学药学院,甘肃兰州730000
摘 要:
建立高效液相色谱法测定天然食品保鲜剂（NFP-3）中丹参酮ⅡA含量的方法,为有效控制配方的质量奠定方法学基础。色谱条件为C18色谱柱（250mm×4.6mm,5μm）,甲醇-1%醋酸缓冲液（85∶15）为流动相,检测波长254nm,流速1.0mL/min,柱温30℃。丹参酮ⅡA对照品在1.0～50μg/mL（R=0.9997）呈良好线性关系。本法简便、准确,线性范围宽,可用于NFP-3中丹参酮ⅡA的质量控制。[著者文摘]
关 键 词:
文章出处:
《食品工业科技》-2007年28卷7期,243 -210-211,243页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1002-0306（2007）07-0210-03
Determination of Tanshinone Ⅱ A in natural food preservatire by HPLC method
Abstract:
A determination method of Tanshinone Ⅱ A in natural food preservative （NFP -3） by HPLC was established, so as to control the quality of the extracts. The chromatographic conditions were： C18 column （250mm×4.6mm, 5μm, Phoxmen）, the methanol-1% acetic acid solution （85：15） as mobile phase with 1.0mL/min flow rate, the detected wavelength selected as 254nm, and the column temperature set at 30℃. The result showed that Tanshinone Ⅱ A in 60% ethanol showed good linear relationship within 1.0-50μg/mL （R=0.9997）. The method is simple, accurate and of wider linear range, and can be used for controlling the quality of the NFP-3 preservative.[著者文摘]
Key words:
natural food preservative; Tanshinone Ⅱ A; HPLC; content determination
基金资助:
科技部西部开发科技行动项目（2002BA901A29）.
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