学术
摘 要:
蛋白质指纹图谱技术是近五年发展起来的实验室诊断新技术，它具有操作较简便、多样本检测、检测快速、灵敏性和特异性高等优点，是实验室诊断技术革命性的进展。蛋白质指纹图谱技术在医学领域中，主要用于多种疾病，如肿瘤的早期检测，对肿瘤早期检测的敏感性和特异性均在80％左右。以蛋白质指纹图谱技术为基础的免疫组质谱检测是在一个抗体组上同时捕获多个生物标志，并对捕获的变异或修饰的生物标志进行质谱精确分析。蛋白质指纹图谱技术及免疫组质谱检测技术特别对评估传统肿瘤标记物阴性的恶性肿瘤有意义。而且具有在分子或基因水平早期诊断的特点。蛋白质指纹图谱技术是一项发展前景非常好的诊断技术，具有广阔的临床应用前景。[著者文摘]
文章出处:
《基础医学与临床》-2007年27卷2期 -134-142页
分 类 号:
文献标识码:
A
文章编号:
1001-6325（2007）02-0134-09
Research advance of protein fingerprinting technology in laboratory diagnosis and clinical medicine
XU Yang （Wu Jieping Medical Foundation, Beijing 100062, China）
Abstract:
Protein fingerprinting technology （PFT） is cent five years. It has advantages of simple operation, a novel technology for laboratory diagnosis developed in retesting quickly, high sensitivity and specificity. It is a revolutional progress for laboratory diagnosis. The application of PFT in medical field is mainly for the detection of diseases. The sensitivity and specificity in cancer detection are about 80%. Immunomic mass spectrometry （IMS） is a novel technology using the combined group antibodies for capture multi biomarkers and applying mass spectrometry to precisely analyze the modification or isoforms of the biomarker in single platform, whereas traditional assay could not be able to identify the variation of biomarkers. PFT and IMS have significantly influenced in cancer early detection, especially to evaluate cancers which did not express traditional tumor markers like AFP, CEA, etc. PFT and IMS have characteristics of early detection in the molecular and gene level. PFT and IMS are diagnostic technologies with bright future and potential applications.[著者文摘]
Key words:
protein fingerprinting technology; laboratory diagnostic technology; immunomic mass spectrometry
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