学术
人脐血间充质干细胞体外诱导分化为神经元样细胞
徐虹[1] 马希峰[2] 阮丽荣[3] 杨波[4] 杜英[3]
[1]河南省肿瘤研究所,河南郑州450003 [2]郑州大学第二附属医院骨科,河南郑州450003 [3]郑州大学基础医学院微生物与免疫学教研室,河南郑州450052 [4]郑州大学第一附属医院神经外科,河南郑州450052
摘 要:
目的 从人脐血中分离单个核细胞（MNCs）、体外培养扩增间充质干细胞（MSCs）并研究其生物学特性和定向诱导能力。方法 无菌条件下采集脐血，肝素抗凝，分离单个核细胞。用DMEM／F12培养基进行纯化和扩增培养，分别向神经元样细胞诱导，获取MSCs，显微镜下观察它的形态、尼氏体染色；取扩增2、5、7代的MSCs，免疫细胞化学法检测nestin表达和神经元样细胞特异性标记。结果 诱导扩增后的MSCs尼氏体染色阳性，第2、5、7代MSCs的nestin的阳性表达率分别为（51．2±3．2）％、（34．6±2．7）％、（11．3±3．3）％；神经元特异性烯醇化酶（NSE）在原代细胞无表达，而在2、5、7代MSC的阳性表达率分别为（11．4±2．3）％、（21．78±3．1）％、（40．7±3．4）％。结论 人脐血MSCs具有神经干／祖细胞的特性，在一定条件下能向神经元样细胞分化。[著者文摘]
文章出处:
《基础医学与临床》-2007年27卷3期 -298-301页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1001-6325（2007）03-0298-04
Human umbilical cord blood mesenchymal stem cells differentiated into neuron-like cells in vitro
XU Hong , MA Xi-feng, RUAN Li-rong, YANG Bo, DU Ying （ 1. Henan Tumor Institute, Zhengzhou 450003 ; 2. Department of orthopaedics,the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450003 ; 3. Department of Microbiology and Immunology, College of Bisic Medical Sciences, Zhengzhou University, Zhengzhou 450052 ; 4. Department of Neurosurgery,the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China）
Abstract:
Objective The mononuclear cells （MNCs） were cultivated and expanded into mesenchymal stem cells （MSCs） from human umbilical cord blood, and the purpose of this study was to explore the biological characteristics and induced differentiation ability in vitro. Methods Human umbilical cord blood samples were obtained and the mononuclear cells were isolated from it, then inoculated the MNCs into 25-mm culture flasks containing DMEM/F12 medium. The morphology was observed under microscope. Nissl body staining was used, The passage 2, 4, 7 of the expanded MSCs were induced to differentiate to neuron-like cells. The expressions of nestin and neuron-specific enolase （NSE） on the treated cells were detected by immunocytochemical method. Results Nissl body staining was positive ; Nestin expression was found in （51.2 ± 3.2） % of the second, （34. 6± 2. 7 ） % of the fifth, （ 11.3 ± 3.3 ） % of the seventh passage of MSCs; NSE expression was found in （ 11.4 ± 2. 3 ） % of the second, （21.78 ± 3. 1 ） % of the fifth, （40. 7 ± 3.4） % of the seventh passage of MSCs. Conclusion Cord blood MSCs possess some features of neural stem cells, and have the capacity to differentiate into neuron-like cells under proper conditions.[著者文摘]
Key words:
cord blood; mesenchymal stem cells; neuron-like cell; induction differentiation
基金资助:
河南省医学科技创新人才工程项目（2005018）
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