学术
摘 要:
利用WL营养培养基对分离自昌黎地区赤霞珠葡萄品种的114株酵母菌进行了聚类分析，共得到5个培养类型，并采用5．8 SrDNA—ITS区域RFLP分析对从5个培养类型中选出的27株酵母菌进行了分子生物学鉴定，结果获得了4种不同的酶切图谱。17株被鉴定为Hanseniaspora uvarum，6株被鉴定为Saccharomyces cerevisiae，并对其余4株也进行5．8 SrDNA—ITS区域RFLP分析。[著者文摘]
文章出处:
《酿酒科技》-2007年12期 -17-20页
栏目信息:
文献标识码:
A
文章编号:
1001-9286（2007）12-0017-04
Isolation and Molecular Biology Identification of Yeasts Related to Cabernet Sauvignon
JIAO Hong-ru, LIU Shu-wen, ZU Xian-sheng , CHANG Ya-wei （College of Enology, Northwest A＆F University, Yangling, Shanxi 712100, China）
Abstract:
114 yeast strains of Cabernet Sauvignon from Changli region were under cluster analysis by the use of WL nutrient culture medium and 5 culture types in total were obtained. 27 strains were selected from the above five types for molecular biology identification with the amplify of the 5.8SrDNA-ITS region by PCR （analyzing restriction fragement length polymorphisms of the region） and four different restriction patterns were found. As a result, 17 strains were identified as Hanseniaspora uvarurn, 6 strains were identified as Saccharomyces cerevisiae and the other 4 strains were also identified with the same method.[著者文摘]
Key words:
microbe; Cabernet Sauvignon; yeast; molecular biology identification; 5.8 SrDNA-ITS ;RFLP
基金资助:
陕西名农业科技攻关项目,项目编号：2005K02-G02.
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