学术
摘 要:
选用低能N^＋离子注入酒精酵母AY-15，研究其诱变效应。结果表明，AY-15菌株存活率曲线是典型的“马鞍型”剂量-效应曲线，“马鞍型”区域内具有较高的突变率。经过N^＋离子注入处理后，最终获得1株亮氨酸缺陷型菌株A713，与出发菌株相比，该菌株异戊醇降低39．85％，高级醇总量降低33．62％，发酵性能基本保持不变。通过耐性试验表明，A713耐酒精度达18％，耐盐度为14％，38℃高温发酵性能较30℃变化不大。[著者文摘]
文章出处:
《酿酒科技》-2008年2期,26 -17-21,26页
栏目信息:
文献标识码:
A
文章编号:
1001-9286（2008）02-0017-05
Screening of Saccharomyces cerevisiae Strains with Low Yield of Higher Alcohols by Ion Implantation
WANG Peng-yin, HAO Xin, GUO Xue-wu and XIAO Dong-guang （Tianjin Industrial Microbiology Key-lab, Tianjin University of Science and Technology, Tianjin 300457, China）
Abstract:
The mutagenic effects ofSaccharomyces cerevisiae AY-15 induced by low energy N^＋ ion implantation were investigated. The experimental results showed that the survival rate curve of AY-15 strain took a ＂saddle＂ shape, and the ＂saddle＂ region-the unique mutation effects of N^＋ion implantation-had got better induced mutation action. After N^＋ ion treatment to the parent strain AY-15, one Leu-strain named A713 was finally obtained. Compared with the original strain, the amount ofisoamyl alcohol and total higher alcohols were decreased by 39.85 % and 33.62 % respectively, however, other fermentation characteristics were almost invariable. Through the comparison of the ethanol tolerance and osmo-tolerance among all strains, A713 strain had exceptional ethanol tolerance capability as ethanol concentration was 18 %, moreover, it had satisfactory osmo-tolerant capability as NaCl concentration was 14 %. In addition, the thermo-tolerance test indicated that there was little change in fermenting performance for A713 strain as the temperature was at 38 ℃ or at 30℃.[著者文摘]
Key words:
microbe; yeast; ion implantation; mutation; higher alcohols; ethanol tolerance; osmo-tolerance;thermo-tolerance
基金资助:
国家863计划项目资助（2006AA020101）.
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