学术
摘 要:
冷冻干燥是乳酸菌和益生菌干燥的主要方式，冷冻和干燥2个过程都会影响菌体细胞的存活率，探讨冷冻和干燥过程对长双歧杆菌的损伤作用有利于指导高存活性益生菌粉的生产。采用透射电镜观察菌体在冷冻干燥过程中的微结构，通过溢出胞外的β-半乳糖苷酶活性评价冷冻和干燥后细胞膜通透性的变化，通过测定冷冻和干燥后胞内Na^＋和K^＋浓度的变化研究离子效应的影响。研究结果表明，长双歧杆菌在冷冻干燥过程中的形态、细胞膜通透性和胞内离子浓度变化程度随冻结时间的延长而加剧；冷冻干燥过程使菌体出现细胞质浓缩、质壁分离、菌体皱缩以及破损现象；冷冻干燥使细胞膜通透性增大，使胞内Na^＋、K^＋浓度显著下降，并伴随比例失调。[著者文摘]
文章出处:
《食品与发酵工业》-2007年33卷12期 -32-35页
栏目信息:
Damage of Bifidobacterium longum by Freeze-drying
Liu Lifeng, Meng Xiangchen （The Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China）
Abstract:
Freeze-drying is the major way to produce lactic acid bacteria and probiotic powders, and both technique can influence cell survival. In order to evaluate the damage of Bifidobacterium longum by freezedrying, factors such as microstructure, β-galactosidase activity and concentration of Na^＋ and K^＋ were determined in this experiment. The results showed that microstructure, β-galactosidase activity and concentration of Na^＋ and K^＋ were increased during freezing and drying. Other factors changed during the freezing and drying include concentration of cytoplasm, separation between cytoplasm and cellular wall, shrinking and damage of cell, increasing of cell membrane permeability, decreasing and disparity of Na^＋ and K^＋ concentration.[著者文摘]
Key words:
freeze-drying; Bi fidobacterium longum ; damage
基金资助:
黑龙江省自然科学基金重点项目资助（ZJN03-31）.
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