学术
转ATP／ADP转运蛋白基因籼稻的初步研究
黄伟奇[1] 胡昌泉[1] 苏军[1] 朱祯[2] 林文雄[3] 王锋[1]
[1]福建省农业科学院农业遗传工程重点实验室,福建福州350003 [2]中国科学院遗传与发育生物学研究所,北京100101 [3]福建农林大学生命科学学院,福建福州350002
摘 要:
ATP／ADP转运蛋白（AATP）在质体膜上负责外源ATP的转人，直接关系到质体内ATP浓度，从而可能影响质体内相关的一些重要代谢途径（如淀粉合成）．本研究利用4个转基因结构（水稻aatp正反义转基因结构和菊芋aatp正反义转基因结构），采用农杆菌介导法获得了113个转基因水稻克隆．PCR、Southern Blot验证和后代遗传分析表明，外源基因多数以较低拷贝数整合到水稻基因组中．RT—PCR相对定量检测分析表明，插入基因在水稻中高水平表达，且与内源aatp基因在转录水平上存在复杂的相互干扰，反义植株aatp的转录水平比正义株系的低．[著者文摘]
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文章出处:
《福建农林大学学报：自然科学版》-2007年36卷5期 -491-495页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1671-5470（2007）05-0491-05
Preliminary studies on transgenic indica rice with four ATP/ADP transporter constructs
HUANG Wei-qi, HU Chang-quan , SU Jun , ZHU Zhen , LIN Wen-xiong, WANG Feng （ 1. Fujian Key Laboratory of Genetic Engineering for Agriculture, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China; 2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 3. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China）
Abstract:
ATP/ADP translocator protein （AATP） , which resides on the membrane of plastids, is charged of the counter-exchange of cytoplasm ATP with inner ADP, thus relating to several important ATP-depended metabolic pathways inside plastids （such as starch synthesis） , by its influence to the concentration of plastidic ATP. In this paper, four constructs of either sense or antisense cDNA （ a pair for os-aatp from Oryza sativa L. and the other for ht-aatp from Helianthus tuberosus L. ） were introduced into rice Minghui 86 （ O. sativa L. indica） , mediated by Agrobacterium. PCR, Southern Blot validation and genetic analysis on transgenic plants against Hygromycin suppression were performed to showed that exogenous genes were most integrated into rice genome with lower copies. At mRNA level, transcript of inserted genes and their interfering with that of endogenous aatp gene were confirmed by RT-PCR. AATP mRNA concentrations in all antisense lines were lower than that in their sense counterparts.[著者文摘]
Key words:
transgene; indica rice （Oryza sativa L. ） ; starch; ATP/ADP transporter
基金资助:
福建省自然科学基金资助项目（C0410039,B0620003）.
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