学术
高效液相色谱串联质谱法测定动物组织中林可酰胺类和大环内酯类抗生素残留
岳振峰[1] 陈小霞[2] 谢丽琪[1] 吉彩霓[1] 华红慧[1]
[1]深圳出入境检验检疫局食品检验检疫技术中心,深圳518067 [2]深圳大学教务处,深圳518060
摘 要:
建立了动物组织样品中洁霉素、氯洁霉素、红霉素、螺旋霉素、交沙霉素、泰乐菌素、竹桃霉素等7种林可酰胺类及大环内酯类药物多残留的液／质联用确证方法。用乙腈萃取样品中7种林可酰胺类及大环内酯类抗生素，然后用正己烷脱脂，旋转蒸发仪浓缩，以Luna C18（2）色谱柱为分离柱，在正离子模式下以电喷雾电离串联质谱仪进行测定。方法的检出限为0．05—5．3μg／kg。在20、50、200μg／kg 3个浓度水平进行验证实验，方法的线性范围为20-200μg／kg；总体平均回收率为70．3％～102％；相对标准偏差为2．4％～16．6％。本方法简便、快速、准确，各项技术指标满足国内外法规的要求，可用于动物组织样品中林可酰胺类及大环内酯类抗生素残留的确证检测。[著者文摘]
文章出处:
《分析化学》-2007年35卷9期 -1290-1294页
栏目信息:
分 类 号:
Determination of Residues of Lincosamides and Macrolides in Animal Tissue by High Performance Liquid Chromatography Tandem Electrospray Mass Spectrometry
Yue Zhen-Feng , Chen Xiao-Xia, Xie Li-Qi , Ji Cai-Ni, Hua Hong-Hui （1. Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People＇s Republic of China, Shenzhen 518067 ;2. Educational Department, Shenzhen University, Shenzhen 518060）
Abstract:
A confirmative method was developed with high performance liquid chromatography tandem electrospray mass spectrometry （LC/MS/MS） to detect 7 residues of lincosamides and macrolides in animal tissues, which include lincomycin, clindamycin, tylosin, spiramycin, josamycin, erythromycin and oleandomycin. In the method, the 7 residues were extracted with acetonitrile, cleaned with hexane, and concentrated by rotary evaporator. Then the reconstituted sample solution was analyzed by LC/MS/MS in positive ion mode, with Luna C18 （2） column as the separation column. The limits of detection of the method are between 0.05 μg/kg and 5.3 μg/kg. The method is validated at 20 μg/kg, 50 μg/kg and 200 μg/kg level. The validation results were as follows： the linear range was from 20 μg/kg to 200μg/kg; the overall recovery varies from 70.3% to 102%, and the relative standard deviation （RSD） was between 2.4% and 16.6%. The method is simple, rapid, and accurate, and its performance can meet the requirements of the domestic and international legislation. The method adapts to simultaneously confirm residues of lincosamides and macrolides in animal tissue.[著者文摘]
Key words:
High performance liquid chromagography tandem mass spectrometry, animal tissue, lincosamides, macrolides
基金资助:
本文系深圳出入境检验检疫局科研基金资助项目（No.SZK03-2003）
更多>>免费文章
cqvip.com