学术
摘 要:
为研究钙稳态失衡在LaCl3诱导MDCC-MSB1细胞凋亡中的作用，将MDCC-MSB1细胞常规培养于RPMI-1640培养液中，加入终浓度为0．5、1．0、1．5、2．0、2．5、3．0、3．5和4．0mmol／L的LaCl3，继续培养24h后，应用MTT法检测细胞增殖抑制率，DNA Ladder法和TUNEL法检测细胞凋亡，以Fura-2为荧光探针检测细胞内[Ca^2＋]i的变化。在LaCl3浓度为0．5-4．0mmol/L范围内，细胞的增殖抑制率增加，细胞凋亡数量和细胞内[Ca^2＋]i呈升高趋势，并呈剂量一效应关系。结l果表明，LaCl3能抑制MDCC—MSB1细胞的增殖，并可能通过改变[Ca^2＋]i而诱导其发生凋亡。[著者文摘]
文章出处:
《中国家禽》-2008年30卷3期 -9-12页
栏目信息:
相关文章:
Effect of the Disequilibrium of Calcium Homeostasis on the Apoptosis of MDCC-MSB1 Cells Induced by Lanthanum Chloride
ZHAO Lan,LI Wenjun,WANG Jintao,XU Shiwen （College of Animal Medicine,Northeast Agricultural University,Harbin,Heilongjiang 150030）
Abstract:
To study the effect of the disequilibrium of calcium homeostasis on the apoptosis of MDCC-MSB1 cells induced by lanthanum chloride,MDCC-MSB1 cells was conventionally cultured in RPMI-1640 for 24 h,which was added with concentration are 0.5,1.0,1.5,2.0,2.5,3.0,3.5 and 4.0 mmol/L, to utilize MTT to detect the cell multiplication inhibition ratio,use DNA Ladder and TUNEL to detect apoptosis,use the Fura-2/AM as the probe to detect the calcium ion within nerve cells concentration （[Ca^2＋ ]i）. LaCl3 concentration is in the confine of 0.5 to 4.0 mmol/L,cell multiplication inhibition ratio was increasing,apoptosis and [Ca^2＋]i were offering an increasing tendency,which present dose- effectiveness relationship. The results showed that LaCl3 could restrain the proliferation of MDCC-MSB1 cells and derive apoptosis happens by changing[Ca^2＋]i.[著者文摘]
Key words:
lanthanum chloride ; MDCC-MSB 1 ; DNA damage ; calcium homeostasis ; apoptosis
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