学术
高功率微波辐射对大鼠脑HIF-1α表达影响
王旭 胡向军 彭瑞云 王水明 高亚兵 王丽峰 吴振宇 魏丽 马俊杰 胡文华 苏镇涛
军事医学科学院放射与辐射医学研究所实验病理研究室,北京100850
摘 要:
目的探讨高功率微波（high power microwave,HPM）辐射后大鼠不同脑区低氧诱导因子1α（hypoxia in-ducible factor-1 alpha,HIF-1α）的改变及其意义。方法60只Wistar雄性大鼠于0,30和100mW/cm2HPM辐射后6h,1,3和7d时活杀取大脑皮层和海马,采用苏木素-伊红染色观察形态改变,采用免疫组织化学、原位杂交和图像分析等技术检测大鼠不同脑区HIF-1α蛋白和mRNA表达改变。结果对照组大鼠皮层和海马毛细血管形态规则,未见毛细血管间隙增宽;30和100m Wcm2组大鼠皮层和海马脑辐射后6h～1d毛细血管周隙增宽,3d达高峰,7d后恢复;对照组HIF-1α蛋白于皮层和海马神经细胞呈弱阳性,30和100m Wcm^2组皮层和海马神经元和胶质细胞核HIF-1α于辐射后6h～1d呈阳性,其后呈弱阳性;HIF-1α mRNA改变规律与其蛋白变化相同。结论脑内神经元的损伤和修复过程中HIF-1α表达增加,参与了毛细血管通透性升高的过程。[著者文摘]
文章出处:
《中国公共卫生》-2007年23卷10期 -1161-1163页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1001-0580（2007）10-1161-03
Expression and significance of hypoxia inducible factor-1 alpha in rat brain after HPM exposure
WANG Xu, HU Xiang-jun, PENG Rui-yun, et al. （Institute of Radiation Medicine, Acadmey of Military Medical Sciences ,Beijing 100850, China ）
Abstract:
Objective To investigate the expression and significance of HIF-1α in the brain of rats after HPM exposure. Methods 60 Wistar rats were sacrificed after the exposure of 0, 30 and 100 mW/cm^2 H＇PM at 6h, ld, 3d and 7d to obtain the brains. HE stain was used to investigate the morphous of blood capillary; Immunohistochemistry, in situ hybridization and quantitative analysis methods were used to investigate the HIF-1α protein and mRNA expression. Results The morphous of capillary in sham exposure groups were regular and no widen gap were found; more widen gaps were found in 30 and 100 mW/cm^2 exposure group. The expression of HIF-1α protein was weakly positive in sham groups, which increased in 30 and 100 mW/cm^2 groups during 6 h to 1 d, and then decreased. Conclusion The increased expression of HIF-1α participate in the injury and reparative process in the brain.[著者文摘]
Key words:
high power microwave（HPM） ; brain; blood capillary; permeability; HI F 1
基金资助:
全军“十一五”医药卫生专项项目（06Z065）
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