学术
采用CUSOC工艺精制凝胶多糖
赵振刚[1] 陈山[2] 卢家炯[2] 于淑娟[1]
[1]华南理工大学轻工食品工程学院,广东广州510640 [2]广西大学糖业工程技术研究中心,广西南宁530004
摘 要:
为得到高纯度的凝胶多糖,采用分级膜（30 ku,100ku）超滤同时耦合溶析结晶的方法——循环超滤-溶析结晶（CUSOC）法对凝胶多糖粗品进行了分级纯化,制备出了纯度分别达96.5%和93.3%的凝胶多糖产品,并对100ku膜截留液所精制的多糖（CUD3）的组成进行了初步分析.结果表明,整个工艺过程的多糖截留率都在95%以上,多糖聚合物总收率约为93.10%.紫外光谱和纸层析鉴定表明,CUSOC方法能有效除去大部分小分子色素及核酸类杂质,凝胶多糖的水解物为单一葡萄糖.红外光谱分析表明,该多糖属β-D-糖苷键构型.[著者文摘]
文章出处:
《华南理工大学学报：自然科学版》-2007年35卷4期 -46-51页
栏目信息:
文献标识码:
A
文章编号:
1000-565X（2007）04-0046-06
CUSOC Process for Separation and Purification of Curdlan
Zhao Zhen-gang,Chen Shan, Lu Jia-jiong,Yu Shu-juan（1. School of Light Industry and Food Sciences, South China Univ. of Tech. , Guangzhou 510640, Guangdong, China; 2. Sugar Engineering Research Center, Guangxi Univ. , Nanning 530004, Guangxi, China）
Abstract:
In order to obtain curdlan with high purity, a new technology named Cyclic Uhrafihration-Solvating-Out Crystallization （CUSOC） was developed to separate and purify crude curdlan. This technology coupled a fractional uhrafiltration separation system as well as two portions （is 30 -100ku, more than 100ku） , with the solvating-out crystallization, by which curdlan with the purities of 96.5% and 93.3% , were obtained. The composition of CUD3 obtained with 100ku membrane was then preliminarily analyzed. The results show that, in the CUSOC process, the rejection rates are all up to 95% when using 30ku and 100ku membrane, and the total recovery of polysaccharides in the whole process reaches about 93.10%. It is also indicated by UV spectrum and paper chromatography that most pigment and nucleic acid impurity can be effectively removed in the process of CUSOC, and that the hydrolysis of CUD3 is a mono-ingredient only composed of glucose. Moreover, the IR spectrum reveals that CUD3 possess a β-D- glycoside linkage.[著者文摘]
Key words:
curdlan ; separation ; purification ; uhrafihration ; solvating-out crystallization ; process coupling
基金资助:
广西区科技攻关项目（0630004-2A）
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