学术
抗菌肽Cecropin P1基因真核表达载体的构建
李建霞[1,2] 苗向阳[2] 丁兆忠[2] 任慧英[1,2] 于忠娜[1,2]
[1]莱阳农学院动物科技学院,城阳266109 [2]中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京100094
摘 要:
选择大肠杆菌偏爱密码子，人工设计合成3条抗菌肽Ceeropin P1基因寡核苷酸片段，通过PCR扩增得到抗菌肽Ceeropin P1基因的全序列，并将其克隆到T—easy载体上，经双酶切重组于真核表达质粒peDNA3．1（＋）上，经酶切鉴定和序列分析，抗菌肽Ceeropin P1基因表达载体构建成功。抗菌肽Ceeropin P1基因表达载体的成功构建，为进一步研究其抗菌活性、抗菌机理及应用等打下基础。[著者文摘]
文章出处:
《中国畜牧兽医》-2007年34卷9期 -58-60页
分 类 号:
文献标识码:
A
文章编号:
1671-7236（2007）09-0058-03
Construction of Eukaryotic Expression Vector of Antimicrobial Peptide Gene Cecropin P1
LI Jian-xia , MIAO Xiang-yang, DING Zhao-zhong , REN Hui-ying , YU Zhong-na （1. Animal Science and Technology Department of Laiyang Agriculture College, Chengyang 266109,China ; 2. Institute of Animal Sciences,Chinese Academy of Agriculture Sciences, State Key Laboratory of Animal Nutrition, Beijing 100094,China）
Abstract:
Three gene fragments were designed and synthesized according to the preference of E. coll. The gene of Cecropin P1 was amplified by PCR, The target gene fragment modified was cloned into vector T-easy, and then inserted into expression plasmid pcDNA3.1 （＋）,the expression vector construction was completed. It was confirmed that the rombinant plasmid was successfully constructed by restriction endonuclease analysis and DNA sequencing, This may lay a foundation in research of antimicobial activities and antimicrobial mechanism in the gene of Cecropin P1.[著者文摘]
Key words:
Cecropin PI;construction of expression vector; antimicrobial peptide
基金资助:
中国农业科学院创新基金资助项目（2004-院-1）.
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