学术
青贮料中肠球菌的分离与筛选
姜艳美[1,2] 王加启[1] 邓露芳[1] 雒秋江[2] 卜登攀[1]
[1]中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京100094 [2]新疆农业大学,乌鲁木齐830052
摘 要:
本试验从青贮料中分离肠球菌并对分离菌株进行初步鉴定与筛选，通过正交试验设计研究不同温度、初始pH和瘤胃液含量对3株分离菌生长的影响，筛选出适合作为奶牛饲用微生物的菌株。结果表明，3株分离菌的最佳培养条件相同为：培养基含葡萄糖2％、蛋白胨1％、瘤胃液20％、初始pH为5．8，接种量为3％，装液量为40ml，36℃培养24h。在该条件下，3株分离菌的生物量分别为1．9907、1、2963和1．2752。选择生物量最高的1号菌送中科院微生物所鉴定，结果为屎肠球菌。[著者文摘]
文章出处:
《中国畜牧兽医》-2008年35卷1期 -19-21页
分 类 号:
文献标识码:
A
文章编号:
1671-7236（2008）01-0019-03
Isolation and Screening of Enterococcus From Silage
JIANG Yan-mei , WANG Jia-qi , DENG Lu-fang , LUO Qiu-jiang , BU Deng-pan （1. Beijing Institute of Livestock Veterinary, Chinese Academy of Agricultural Science, State Key Lab of Animal Nutrition, Beijing 100094,China;2. Xinjiang Agricultural University, Urumqi 830052 ,China）
Abstract:
This experiment was preliminary identification and screening the Enterococcus isolated from silage. Three isolates were influenced by three factor： temperature, initial pH value and concentration of rumen liquid, studied by orthogonal design. Screening the potential probiotic strains for dairy cow. The results showed that three isolates have the similar best culture condition： medium contain 2 % glucose, 1% peptone, 20% rumen liquid, initial pH value 5.8, 3 % inoculation,40 ml liquid capacity , fermentation temperature was 36℃, fermentation time was 24 h. Under this condition,the biomass of three isolates were 1. 9907,1. 2963 and 1. 2752. Number 1 isolates which have the max biomass was identified to be Enterococcus Faeeium by Institute of Microbiology , Chinese Academy of Sciences.[著者文摘]
Key words:
silage; enterococcus ;isolation ; screening
基金资助:
国际科技合作重点项目：“奶牛安全饲用微生物及其人工瘤胃评价技术研究”（2004DFA06700）和国家科技支撑计划“反刍、水产等动物配合饲料生产技术集成与产业化示范”（2006BAD04A08）.
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