学术
摘 要:
马铃薯软腐病是威胁马铃薯块茎的细菌性病害之一,为了提高马铃薯的品质与产量,减少贮藏期马铃薯块茎的腐烂,对马铃薯进行软腐病检测与鉴定是十分必要的。利用16SrDNA PCR对马铃薯软腐病原菌的进行检测,证实16SrDNA PCR检测技术是可行可靠的。在对PCR引物的筛选中,要根据所检测的对象进行有效、合理的设计,才能达到快速有效的目的。以马铃薯软腐病菌为代表,利用16SrDNA PCR进行鉴定,达到了预期的目的,为病原菌的快速鉴定方法提供了依据。[著者文摘]
文章出处:
《中国马铃薯》-2007年21卷4期 -206-208页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1672-3635（2007）04-0206-03
Test for Soft Rot Pathogenic Bacteria on Potato via the Method of 16SrDNA PCR
Wang Min, Zhang Ru （ 1. Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China; 2. College of Agromomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China ）
Abstract:
The soft rot of potato is one of the bacterium diseases influencing potato production. In order to improve the quality and yield of potato and decrease the rotten tubers, it is necessary to test the pathogen responsible for the soft rot effectively in potato. The test of soft rot pathogenic bacteria on potato via the method of 16S rDNA PCR was proven to be feasible and reliable. In the process of primer screening, the right primer can be reached by effective design based on the target tested. In the case of soft rot, 16S rDNA PCR could be used to test the pathogen, and this work lay a sound foundation for rapid detection of soft rot in potato.[著者文摘]
Key words:
potato; 16SrDNA; PCR; pathogen
基金资助:
教育部国际合作项目：应用基因工程提高马铃薯加工型品种的抗逆性
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