学术
猪GATA-3基因的电子克隆、表达及其分子进化分析
于浩[1,2] 李景芬[4] 李丽[1] 宋跃芬[1] 杨秀芹[1] 丁琳琳[1] 刘娣[1,3]
[1]东北农业大学动物科技学院,哈尔滨150030 [2]吉林大学农学部畜牧兽医学院,长春130062 [3]黑龙江省农科院,哈尔滨150086 [4]湖州师范学院生命科学院,湖州313000
摘 要:
基于猪的表达标签数据库电子克隆了猪的GATA-3基因，并通过RT-PCR验证了猪的GATA-3基因的核苷酸序列。测序结果显示猪的GATA-3基因的核苷酸长度由1,760bp个碱基组成，包括1，335bp的开放阅读框，编码产物为由444个氨基酸残基组成的多肽。通过半定量RT-PCR检测了GATA-3 mRNA在大白猪的各个组织的表达情况。GATA转录因子家族通常有2个Ⅳ型锌指蛋白结构，根据Ⅳ型锌指蛋白结构序列，使用Mega3．1软件构建了分子进化关系树。系统发育分析表明所有的脊椎动物的GATA转录因子都起源于共同的祖先，拓扑结构也表明进化过程中有多种事件发生，包括基因复制和Ⅳ型锌指蛋白结构域重组，根据所得数据有利于进一步了解GATA家族基因趋同和趋异的进化途径。[著者文摘]
文章出处:
《遗传学报》-2007年34卷11期 -994-1000页
栏目信息:
分 类 号:
In Silico Cloning, Expression and Phylogenetic Analysis of Pig GATA-3 Gene
Hao Yu, Jingfen Li , Li Li , Yuefen Song , Xiuqin Yang , Linlin Ding , Di Liu （1. Animal Science College, Northeast Agricultural University, Harbin 150030, China;2. Institute of Animal Husbandry and Veterinary Medicine, Jilin University, Changchun 130062, China;3. Heilongjiang Agricultural Science Institute, Harbin 150086, China;4, College of Life Science, Huzhou University, Huzhou 313000, China）
Abstract:
Pig GATA-3 cDNA was obtained by reverse transcription polymerase chain reaction （RT-PCR）, using in silico cloning strategy based on pig dbEST. The length of pig GATA-3 cDNA is 1,760 bp containing a 1,335 bp open reading frame （ORF）, which encodes a 444 amino acid protein. Semi-quantitative RT-PCR analysis of GATA-3 mRNA expression was done using the total RNAs from different normal tissues of a large white pig. The GATA-binding family of transcription factors comprised of a subgroup of DNA-binding proteins that both bound the consensus GATA motif and contained the class Ⅳ zinc finger motif. The molecular evolution tree was constructed based on the GATA-3 amino acid sequence and class Ⅳ zinc finger motif using mega 3.1. Phylogeny analysis of GATA factors isolated from vertebrates suggested that the six distinct vertebrate GATAs had descended from a common ancestral sequence, and the topology also suggested multiple modes of evolution including gene duplication and class Ⅳ zinc finger motif recombination. These data helped the authors in illuminating the pathways of divergent and convergent evolution of the GATA-binding family of transcription factors.[著者文摘]
Key words:
GATA-3; RT-PCR; class Ⅳ zinc finger motif; phylogeny
基金资助:
This work was supported by the Heilongjiang Province Tackle Project （No. GB01B104）.
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