学术
糖的浓度和种类对人血小板冷冻干燥保存的影响
周新丽[1] 刘宝林[1] 张绍志[2] 陈光明[2] 祝宏[3] 朱发明[3]
[1]上海理工大学医疗器械与食品学院,上海200093 [2]浙江大学机械与能源工程学院,杭州310027 [3]浙江省血液中心,杭州310006
摘 要:
以海藻糖、蔗糖、麦芽糖、乳糖、葡萄糖作为冻干血小板的保护剂，研究糖的浓度和种类对血小板冻干保存的影响．结果表明，冻干血小板的保存效果随海藻糖浓度的增加而提高，当海藻糖质量分数增加到20％时，非活化血小板的恢复率达到85．7％；对于质量分数均为20％不同种类的糖保护剂，非活化血小板的恢复率都达到70％以上，海藻糖略优于其他几种糖，但它们之间没有显著性差异．[著者文摘]
文章出处:
《上海理工大学学报》-2007年29卷6期 -534-538页
分 类 号:
文献标识码:
A
文章编号:
1007-6735（2007）06-0534-05
Influence of saccharides and their concentrations on freeze-drying of human platelets
ZHOU Xin-li, LIU Bao-lin, ZHANG Shao-zhi, CHEN Guano-ming, ZHU Ho, ZHU Fa-ming （ 1. College of Medical Instrumentation and Foodstaff, University of Shanghai for Science and Technology, Shanghai 200093, China ; 2. College of Mechanical and Energy Engineering,Zhejiang University, Hangzhou 310027, China ; 3. Blood Center of Zhejiang Province, Hangzhou 310006, China ）
Abstract:
Trehalose, sucrose, maltose, lactose and glucose were used as protectants for platelet freeze -drying, the effects of various kinds of saccharides and their concentrations on the viability of freeze-dried platelets were investigated. Raising the concentration of trehalose to 20 %, the recovery rate of nonactived platelets can reach to 85.7%. For various kinds of saccharides with the same concentration of 20 %, the recovery rate of nonactived platelets is above 70 %, trehalose is slightly higher than others, however there is no statistically significant difference among them.[著者文摘]
Key words:
platelets ; freeze-drying ; trehalose
基金资助:
卫生部科学研究基金资助项目（WKJ2005-2-037）;上海市高校选拔培养优秀青年教师科研专项基金资助项目（21035）;上海市曙光计划科学研究基金资助项目（065G47）
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