学术
芸芥发状根的诱导及其植株再生体系的建立
武振华[1,2] 牛炳韬[1] 张涛[1] 祁永和[1] 王新宇[1]
[1]兰州大学生命科学学院,甘肃兰州730000 [2]中国科学院近代物理研究所,甘肃兰州730000
摘 要:
通过发根农杆菌R1000介导的遗传转化建立了芸芥发状根的诱导、培养及植株再生体系，研究了影响发状根诱导及生长的各种因素，通过PCR对所得发状根进行了分子水平的鉴定，结果表明：发根农杆菌R1000的rolB基因已被成功地转入并整合到该发根培养物的基因组中。[著者文摘]
文章出处:
《兰州大学学报：自然科学版》-2007年43卷5期,53 -44-47,53页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
0455-2059（2007）05-0044-05
Hairy roots induction and plant regeneration in Eruca sativa Mill
WU Zhen-hua, NIU Bing-tao, ZHANG Tao, QI Yong-he, WANG Xin-yu （1. School of Life Sciences, Lanzhou University, Lanzhou 730000 China; 2. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China）
Abstract:
The system of induction and culture of hairy roots derived from cotyledon mediated by Agrobac- terium rhizogenes R1000 and the plant regeneration from hairy roots in Eruca sativa Mill were established, and the factors that affect the inducement and growth of hairy roots were also studied. Furthermore, this study testified the hairy roots in molecular level by the PCR analysis. The results of such identification demonstrated that rol B gene of T-DNA from Ri plasmid had been integrated into the genome of the transformed roots.[著者文摘]
Key words:
Eruca sativa Mill; hairy root; agrobacterium rhizogene
基金资助:
国家自然科学基金资助项目（30370709）.
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