学术
弓形虫GRA4和SAG2基因重组BCG疫苗免疫保护性的比较研究
李俊华[1] 吴少庭[1] 翁亚彪[2] 甘燕[1] 刘洪波[3] 刘茂玲[3] 张仁利[1] 高世同[1] 黄达娜[1] 耿艺介[1]
[1]深圳市疾病预防控制中心,深圳518020 [2]华南农业大学兽医学院,广州510642 [3]华中科技大学同济医学院,武汉430030
摘 要:
目的比较弓形虫致密颗粒蛋白4（GRA4）基因和表面抗原2（SAG2）基因的重组卡介苗（BCG）对小鼠的免疫保护效果。方法108只SPF级雌性BALB/c小鼠随机分成6组：PBS组、BCG空白菌组、BCG-空白载体组、BCG-SAG2组、BCG-GRA4组和BCG-SAG2＋GRA4组,每组18只。每鼠分别注射对应液体/疫苗100μl,共2次,间隔2周。接种前尾静脉采血,接种后4、6、8周每组分别剖杀3只,取脾和眼眶血检测细胞因子、IgG与IgM抗体,T淋巴细胞亚群计数,淋巴细胞转化率等。末次免疫后3周,每组剩余小鼠分别腹腔接种RH株弓形虫速殖子50个进行攻击感染,观察各组小鼠存活时间。结果弓形虫SAG2和GRA4重组BCG疫苗均能诱导小鼠产生免疫应答。第4周时,BCG-GRA4＋SAG2免疫组小鼠的CD3＋CD4＋/CD3＋CD8＋的比值最高,为14.06%±1.17%。第6周时,BCG-GRA4＋SAG2免疫组小鼠的IgG抗体水平最高,为0.18±0.02。第8周时,BCG-SAG2免疫组小鼠的IgM抗体水平最高,为0.82±0.05;弓形虫速殖子攻击后,BCG-SAG2组平均存活8.61d,PBS对照组平均存活7.33d,3个免疫组小鼠比其他3组的平均存活时间长1d。结论弓形虫重组BCG疫苗具有一定的免疫保护性。[著者文摘]
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文章出处:
《中国寄生虫学与寄生虫病杂志》-2007年25卷3期 -217-221页
栏目信息:
文献标识码:
A
文章编号:
1000-7423（2007）-03-0217-05
Study on Immuno-effect with GRA4 or SAG2 Gene Recombinant BCG Vaccine of Toxoplasma gondii
LI Jun-hua, WU Shao-ting, WENG Ya-biao, GAN Yan, LIU Hong-bo, LIU Mao-ling, ZHANG Ren-li, GAO Shi-tong, HUANG Da-na, GENG Yi-jie （1 Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China; 2 College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; 3 Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China）
Abstract:
Objective To compare the immuno-protection induced by the recombinant BCG vaccine of Toxoplasma gondii GRA4 gene （rBCG-GRA4） and SAG2 gene （rBCG-SAG2） in BALB/c mice. Methods 108 SPF BALB/c mice were divided into 6 groups： PBS, BCG, rBCG, rBCG-GRA4, rBCG-SAG2 and rBCG-GRA4＋SAG2, each with 18 mice. Each mouse was injected by 100 μl corresponding materials for 2 times. Blood was taken from tail vein before inoculation. 4,6 and 8 weeks after inoculation, spleen was moved and blood was taken from orbit vein of 3 mice from each group for the detection of cytokines, IgG and IgM antibodies, T lymphocyte subgroups and transformation efficiency. 3 weeks after the last inoculation, 9 mice from each group were challenged intraperitoneally with 50 tachyzoites of T.gondii RH strain and their survival time was observed. Results rBCG vaccine of T.gondii induced immune response. The value of CD3^＋ CD4^＋/CD3^＋CD8^＋ of group BCG-GRA4^＋SAG2 was the highest （14.06±1.17） in the 4th week; the IgG titer in the BCGGRA4＋SAG2 group was the highest （0.18±0.02） in the 6th week and the IgM titer in the BCG-SAG2 group was the highest （0.82±0.05） in the 8th week. The average survival time of the mice in BCG-SAG2 group was about 8.61 days after challenged with tachyzoites, and that of the PBS control group, 7.33 days. The average survival time in the 3 immunized groups was one day longer than that of the control. Conclusion The rBCG vaccine of T.gondii shows certain immuno-protection in mice.[著者文摘]
Key words:
Toxoplasma gondii; Dense granule protein 4 （GRA4）; Surface antigen 2 （SAG2）; Recombinant vaccine; Bacillie Calmette-Guérin; Immuno-protection
基金资助:
深圳市科技局基金项目（No.15.1997）
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