学术
麻疯树新基因JcPIP的分子克隆与功能研究
张颖[1,2] 江璐玎[1] 王云霄[1] 牛蓓[1] 陈放[1]
[1]生物资源与生态环境教育部重点实验室（四川大学生命科学学院） [2]海南师范大学生物系
摘 要:
从大戟科耐旱植物麻疯树eDNA中克隆得到了一个麻疯树质膜内膜蛋白（PIP）新基因（JcPIP）。其eRNA在非洲爪蟾卵母细胞（Xenopus oocytes）中异源表达后发现细胞膨胀率扩大了10倍。聚类分析表明，麻疯树PIP蛋白与蓖麻、葡萄和菠菜PIP蛋白在进化上有最近的亲缘关系。半定量RT—PCR研究表明该基因在植株的整个生长发育期都有表达。但干旱胁迫下JcPIP基因表达量没有显著变化。[著者文摘]
文章出处:
《林业科技开发》-2007年21卷6期 -12-16页
栏目信息:
分 类 号:
Cloning and Function of a New Aquaporin Gene （JcPIP） from Jatropha Curcas
ZHANG Ying, JIANG Lu-ding,WANG Yun-xiao, NIU Bei,CHEN Fang（ Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University,610064, Chengdu, China.）
Abstract:
Amplified by PCR with degenerated primers, a new plasma membrane intrinsic protein gene （JcPIP） was cloned from the cDNA of Jatropha curcas. The cRNA of JcPIP was hetero expression in Xenopus oocytes as a water channel and increased swelling rate 10 times higher. Phylogenic analysis indicated that PIPs of Jatropha curcas was relation to Ricinus com- munis, Vitis vinifera and Spinacia oleracea closely. Semi-quantitative PCR analysis showed it was expressed in each phase of Jatropha curcas growth. Furthermore, there were no obviously changes in PIPs expression levels under drought stress.[著者文摘]
Key words:
Aquaporin; Function analysis ; Drought stress
基金资助:
国家自然科学基金面上项目（编号：30670204）;国际合作项目（编号：2003DFB00005）.
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