学术
摘 要:
本文以杜仲（Eucommia ulmoides Oliv．）为材料，进行了带芽茎段的快速繁殖研究。结果表明：以成年树带芽茎段为外植体进行快速繁殖，1a中以4、5月份所采取的腋芽萌发和伸长最好，萌发率达90．0％以上，伸长率达80．0％以上，并且中部和顶部腋芽的萌发效果较基部的好。MS附加6-BA0．10mg·L^-1的培养基诱导腋芽萌发，芽苗在MS附加6-BA2．00mg·L^-1和NAA0．05mg·L^-1培养基上继代增殖，以I／2MS附加IBA1．50mg·L^-1和蔗糖20．00mg·L^-1培养基中诱导生根，生根率达90．0％。[著者文摘]
文章出处:
《吉林林业科技》-2007年36卷4期 -12-16页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1005-7129（2007）04-0012-05
In vitro culture of stem segment with axillary bud of Eucommia ulmoides
LUO Li , ZHAO De - gang （1. Lianyungang Teachers College, Lianyungang 222006,China; 2. Guizhou University, Guiyang 550025, China ）
Abstract:
Rapid propagation of Eucommia ultooides was studied by using stem segment with axillary bud. The results were as follows： The explants taken from the middle and top of adult trees in April and May per year had best sprouting ability and primary growth. The germination rate reached above 90.0%, the elongation ratio reached above 80.0%. After buds sprouting were induced by MS ＋ 6- BA 0. 10 mg·L^-1 , and budlng plants were subeultured for multiplication by MS ＋ 6 - BA 2.00 mg·L^-1 ＋ NAA 0.05 mg·L^-1, then induced rooting by 1/2MS ＋ IBA 1.50 mg·L^-1 ＋ suerose 20.00 mg·L^-1 , the rooting rate reached 90.0%.[著者文摘]
Key words:
Eucommia ultooides ; rapid propagation; stem segment with axiUary bud
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