学术
HCV e2-3pcx融合基因原核表达载体的构建及蛋白的初步表达
李凤梅[1] 刘均洪[1] 任大明[2]
[1]青岛科技大学化工学院,山东青岛266042 [2]复旦大学遗传学研究所遗传工程国家重点实验室,上海200433
摘 要:
构建了丙型肝炎病毒e2-3pcx融合基因的原核表达载体pET28b（＋）-e2-3pcx,并转化到大肠杆菌BL21（DE3）菌株中。IPTG诱导融合蛋白高效表达。经SDS-PAGE电泳分析,结果表明在43 ku处有一条蛋白质特异条带,与预期的目的产物蛋白带大小一致。[著者文摘]
文章出处:
《青岛科技大学学报》-2007年28卷5期 -383-385页
栏目信息:
分 类 号:
文献标识码:
B
文章编号:
1672-6987（2007）05-0383-03
Preliminary Construction and Expression of HCV e2-3pcx Fusion-gene Vector in E.coli
LI Feng-mei;LIU Jun-hong;REN Da-ming（1.College of Chemical Engineering;Qingdao University of Science and Technology;Qingdao 266042;China;2.State Key Laboratory of Genetic Engineering;Institute of Genetics;Fudan University;Shanghai 200433;China）
Abstract:
Prokaryotic expression vector for e2-3pcx fusion-gene of Hepatitis C Virus（HCV） was constructed into pET28b（＋）-e2-3pcx vector,and was transformed into BL21（DE3）.Upon induction by IPTG,e2-3pcx fusion-gene was expressed as a major protein product in the total cellular protein of the recombinant vector compared with all the controls.SDS-PAGE was used to analyze the expression of the fusion protein.The result showed that there was a specific band at about 43 ku in size,which was identical with the expected molecular weight of the fusion protein.[著者文摘]
Key words:
e2 gene;3pcx gene;prokaryotic expression
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