学术
摘 要:
目的 了解近两年武汉地区四家教学医院院内感染阴沟肠杆菌耐药现状，以及去阻遏持续高产AmpC酶和超广谱β-内酰胺酶（ESBLs）及同时表达这两类酶的发生率。方法 收集武汉地区四家医院558株医院内感染阴沟肠杆菌，用Kirby-Bauer法对15种抗菌药物进行药物敏感试验，并用改良的酶提取物三维试验法，检测阴沟肠杆菌去阻遏持续高产AmpC酶，用改良双纸片法检测ESBLs。结果 阴沟肠杆菌仅对亚胺培南、头孢吡肟显示较好的敏感性。三维试验和表型筛选试验表明，558株实验菌中，65（11．6%）株持续高产AmpC酶，135（24．2％）株产ESBLs，28（5．0%）株同时表达这两种酶。结论阴沟肠杆菌对常用抗生素耐药现象较严重，耐药性呈上升趋势。高产AmpC酶和ESBLs的阴沟肠杆菌在四家教学医院的分离率较高。对阴沟肠杆菌进行耐药性监测及去阻遏持续高产AmpC酶和ESBLs检测，能较好了解该地区细菌耐药的现状，为当地临床医生抗生素的使用提供合理建议。[著者文摘]
文章出处:
《现代检验医学杂志》-2007年22卷4期 -6-8页
栏目信息:
文献标识码:
A
文章编号:
1671-7414（2007）04-006-03
Current Status and Resistance of Enterobacter Cloacae in Nosocomial Infection in Wuhan
SHI Jin-ling ,CAI Xuan,YANG Xiang-sheng,PENG Shao-hua （Department of Clinical Laboratory,People＇s Hospital, Wuhan University,Hubei Wuhan 430060 ,China）
Abstract:
Objective To estimate the occurrence of derepressed hyperproduction AmpC β-lactamases,extended-spectrum β-lactamases（ESBLs）and dual enzyme expression ,as well as drug resistance profile in Enterobacter cloacae（E, cloacae）isolates at large hospitals in Wuhan. Methods 558 E. cloacae isolates were collected by a clinical laboratory from four teaching hospitals in Wuhan,China,from 2005 to 2006. Resistance to 15 antibiotics in these isolates was assessed with Kirby-Bauer method. Modified three-dimensional extract test and modification of the double-disk test were used to detect hyperproduction AmpC β-lactamases and ESBLs,respectively. Results All isolates were found to be highly sensitive to Imipenem and Cefepime. Among 558 isolates, 65 （11.6 ~ ）isolates produced hyperproduction AmpC β-lactamases, 135 （24.2 ~ ） iso- lates produced ESBLs,and 28（5.0%）produced both enzymes. Conclusion These results indicate an increased prevalence of resistance among E. Cloacae isolates in Wuhan to either hyperproduction AmpC β-lactamases or ESBLs,or both. It is very important to provide correct suggestions to clinical doctors in antibiotics,and to prevent and control the nosocomial infection.[著者文摘]
Key words:
enterobacter cloacae ;antibiotic resistence ;AmpC β-lactamases ;extended-spectrum β-lactamases
基金资助:
湖北省自然科学基金（2004ABA153）.
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