学术
中国荷斯坦牛BoLA—DRB3．2基因座的PCR—RFLP多态性分析
赵福斌[1,2] 李建斌[1] 王洪梅[1] 胡桂学[2] 李秋玲[1] 仲跻峰[1] 侯明海[1]
[1]山东省农业科学院奶牛研究中心,山东济南250100 [2]吉林农业大学动物科技学院,吉林长春130118
摘 要:
主要组织复合体（Major Histocompatibility Complex，MHC）基因在动物机体免疫系统中具有重要作用，并与多种疾病的抗性或易感性存在相关性。利用限制性内切酶HaeⅢ和RsaⅠ通过FCR-RFLP技术分析了112头中国荷斯坦牛BoLA-DRB3．2基因的多态性。结果表明：所检测牛群中BoLA—DRB3．2基因在两个酶切位点均存在多态性，HaeⅢ酶切位点存在4种等位基因，有4种基因型；RsaⅠ酶切位点存在3种等位基因，有4种基因型。经χ^2适合性检验，所检测牛群中BoLA-DRB3．2基因在两个酶切位点均未达到Hardy-weinberg平衡状态。[著者文摘]
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文章出处:
《家畜生态学报》-2007年28卷5期,30 -25-27,30页
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文献标识码:
A
文章编号:
1004-5228（2007）05-0025-03
Polymorphism Analysis of BoLA-DRB3.2 Gene in Chinese Holstein Cattle by PCR-RFLP
ZHAO Fu-bin , LI Jian-bin , WANG Hong-mei , HU Gui-xue , LI Qiu-ling , ZHONG Ji-feng, HOU Ming-hai. （1. Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100; 2. JilinAgricultural University, Changchun 130118）
Abstract:
MHC（Major Histocompatibility Complex） plays a central role in immune system and associated with affectability or resistance of many diseases. The polymorphism of BoLA-DRB3. 2 in Chinese Holstein cattle was analyzed by PCR-RFLP. The results revealed DRB3.2 gene had extensive po phism,4 alleles and 4 genotypes digested with enzyme HaeⅢ ; 3 alleles and 4 genotypes digested w ymor- thenzyme Rsa Ⅰ. The Genotypic frequency and gene frequency of DRB3.2 gene in China Holstein Cattle was calculated. Statistical results showed that polymorphic locus for HaeⅢ and Rsa Ⅰ of China Holstein DRB3. 2 locus didn＇t fit with Hardy-weinberg equilibrium.[著者文摘]
Key words:
Chinese Holstein ; BoLA-DRB3.2 ; Gene polymorphisms ; PCR-RFLP
基金资助:
国家863计划项目（No.2006AA10ZID9）;山东省农业科学院青年科研基金项目（2005YQ048）;山东省农业良种工程项目（2003-2006）.
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